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Examinee
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DANIEL TSUN-YEE CHIU
 
 

Daniel Tsun-Yee Chiu

Highest Degree

Ph. D. in Biochemistry Univ. of 

Calif at Davis

Areas of Specialty

Clinical Hematology, Wellnest Testing, Free Radical Biomedicine

Office Phone

5097/3505

Lab phone

3708

Research website:

Redox Biology and Redoxomics Research Lab

E-mail

dtychiumail.cgu.edu.tw 

Lab & Research Interest

A.    The Effect of Oxidative Stress on Cellular Function and Human Health

Free radicals like superoxide and nitric oxide are essential for maintaining physiological functions such as phagocytosis, signal transduction and gene regulation. On the other hand, free radicals are also risk factors of ageing (including apoptosis) and degenerative diseases (including cancer, cardiovascular disease, immunosuppresion, brain dysfunction and cataract). Hence, our laboratory employs  G6PD deficient model to investigate how does oxidative stress modulate cellular structure and function. Furthermore, we will address the clinical question whether G6PD-deficient patients are more susceptible to degenerative diseases than normal individuals.

 

Specific Topics:

     1. The effect of oxidative stress on cellular function (G6PD-deficient cell as model system)

Apply RNAi technique to establish G6PD-deficient cell lines and couple with advanced system biology technology (Proteomics and Metabolomics) for analysis of the comprehensive effect of oxidative stress on cellular physiology.

      2. Development of Antioxidants

To screen effective antioxidants from herbal medicine.

 

B.    Research on Hematology

      Research on hematological disorders (including thalassemia and G6PD deficiency) from genomics and proteomics perspectives.

 

C.   Establish Reference Laboratories

     Reference laboratory to assess oxidative stress and antioxidant capacity.

      Develop ultra-sensitive assays such as Coularray coupled with Metabolomics and Bioinformatics to evaluate the level of oxidative stress and assess the role of oxidative stress in degenerative diseases.

Publication

1.      Yang HC, Chen TL, Wu YH, Cheng KP, Lin YH, Cheng ML, Ho HY, Lo SJ* and Chiu DTY*(2013) Glucose 6-phosphate dehydrogenase (G6PD) deficiency enhances germ cell apoptosis and causes defective embryogenesis in Caenorhabditis elegans. Cell Death & Disease (SCI, in press)

2.      Cheng ML, Ho HY, Shiao MS, Chiu DTY* (2013) Characterization of global metabolic responses of glucose-6-phosphate dehydrogenase-deficient hepatoma cells to diamide-induced oxidative stress. Free Radic Biol Med. 54C:71-84(SCI)

3.      Chen YC, Chen CM, Liu JL, Chen ST, Cheng ML, Chiu DTY* (2011) Oxidative markers in spontaneous intracerebral hemorrhage: leukocyte 8-hydroxy-2'-deoxyguanosine as an independent predictor of the 30-day outcome. J Neurosurg115:1184-90 (SCI )

4.    Cheng ML, Shiao MS, Chiu DTY, Weng SF, Tang HY, Ho HY (2011) Biochemical disorders associated with antiproliferative effect of dehydroepiandrosterone in hepatoma cells as revealed by LC-based metabolomics.  Biochem Pharmacol 82:1549-61(SCI )

5.    Chen KH, Cheng ML, Jing YH, Chiu DTY, Shiao MS, Chen JK (2011) Resveratrol ameliorates metabolic disorders and muscle wasting in streptozotocin-induced diabetic rats.  American Journal of Physiology - Endocrinology and Metabolism 301: E853-E863.

6.       Yang HC, Cheng ML, Ho HY, Chiu DTY* (2011) The microbicidal and cytoregulatory roles of NADPH oxidases.  Microbes Infect 13:109~20(SCI)

7.       Lin CJ, Cheng ML, Ho HY, Yu JS, and Chiu DTY* (2010)Impaired dephosphorylation process renders G6PD-deficient cells more susceptible to H2O2-induced apoptosis.   Free Radical Biology & Medicine  49:361~373 (SCI)

8.       Gao LP, Cheng ML, Chou HJ, Yang YH, Ho HY, Chiu DTY* (2009)Ineffective GSH regeneration enhances G6PD knockdown Hep G2 cells to diamide induced-oxidative damage. Free Radical Bio Med 47:529-35 (SCI)

9.       Ho HY, Cheng ML, Weng SF, Leu YL, Chiu DTY* (2009) Antiviral Effect of Epigallocatechin Gallate on Enterovirus 71. J Agr Food Chem 57:6140-7 (SCI)

10.    Wu YH, Cheng ML, Ho HY, Chiu DTY*, Wang TC (2009)Telomerase prevents accelerated senescence in glucose-6-phosphate dehydrogenase (G6PD)-deficient human fibroblasts. J Biomed Sci 16:Articl #18 (online publication) (SCI)

11.    Cheng ML, Chen CM, Li JM, Ho HY, Chiu DTY* (2009)Effect of acute myocardial infarction on erythrocytic glutathione peroxidase 1 activity and plasma vitamin E levels. Am J Cardiol 103:471-5 (SCI)

12.    Chen CM, Liu JL, Wu YR, Chen YC, Cheng ML, Chiu DTY* (2009)Increased oxidative damage in peripheral blood correlates with severity of Parkinson's disease.
Neurobiol Dis 33:429-35 (SCI)

13.    Cheng ML, Ho HY, Lin RF, Chen YC, Chan EC, Chiu DTY* (2008) Clinical relevance of plasma homocysteine levels in Taiwanese patients with coronary artery disease. BioFactors 34:125-34 (SCI)

14.    Cheng ML, Chen CM, Gu PW, Wang YL, Li JM, Ho HY, Chiu DTY* (2008) Oxidative damage markers and antioxidants in patients with acute myocardial infarction and their clinical significance. BioFactors 34:135-45 (SCI)

15.    Ho HY, Cheng ML, Chiu HY, Weng SF and Chiu DTY* (2008) Dehydroepiandrosterone induces growth arrest of hepatoma cells via alteration of mitochondrial gene expression and function. Int J Oncol 33:969-77 (SCI)

16.    Ho HY, Cheng ML, Weng SF, Chang Lo, Yeh TT, Shih SR Chiu DTY* (2008)G6PD Deficiency Enhances Enterovirus 71 Infection. J Gen Virol 89:2080-9SCI

17.    Cheng ML, Chen CM, Gu PW, Ho HY,Chiu DTY*(2008) Elevated levels of myeloperoxidase, white blood cell count and 3-chlorotyrosine in Taiwanese patients with acute myocardial infarction. Clin Biochem 41:554-60 (SCI)

18.    Wu YH, Tseng CP, Cheng ML, Ho HY, Shih SR, Chiu DTY* (2008) G6PD Deficiency Enhances Human Coronavirus 229E Infection. J Infect Dis 197:812-6SCI

19.    Chen CM, Wu YR, Cheng ML, Liu JL, Lee YM, Lee PW, Soong BW, Chiu DTY(2007)Increased oxidative damage and mitochondrial abnormalities in the peripheral blood of Huntington's disease patients. Biochem Bioph Res Co 359:335-40SCI

20.     Ho HY, Cheng ML, Chiu DTY* (2007) Glucose-6-Phosphate Dehydrogenase -From Oxidative Stress to Cellular Functions and Degenerative Diseases. Redox Rep 12:109-18SCI

21.    Ho HY, Cheng ML, Cheng PF, Chiu DTY* (2007) Low Oxygen Tension Alleviates Oxidative Damage and Delays Cellular Senescence in G6PD-Deficient Cells. Free Radical Res 41: 571-9(SCI)

22.    Ho HY, Cheng ML, Wang YH, Chiu DTY* (2006) Flow cytometry for Assessment of the Efficacy of siRNA. Cytom Part A 69:1054-61 (SCI)

23.    Wan GH, Lin KK, Tsai SC, Chiu DTY* (2006) Decreased Glucose-6-phosphate-dehydrogenase (G6PD) Activity and Risk of Senile Cataract in Taiwan. Ophthal Epidemiol 13:109-14 (SCI)

24.    Ho HY, Wei TT, Cheng ML, and Chiu DTY*(2006)Green Tea Polyphenol Epigallocatechin-3-gallate Protects Cells against Peroxynitrite-Induced Cytotoxicity: Modulatory Effect of Cellular G6PD Status. J Agric Food Chem 5:1638-45(SCI)

25.    Tseng CP, Huang CL, Chong KY, Hung IJ, Chiu DTY* (2005) Rapid detection of glucose-6-phosphate dehydrogenase ene mutations by denaturing high-performance liquid chromatography. Clin Biochem 38: 973-80SCI

26.    Ho, HY, Cheng, ML, Chiu DTY* (2005) G6PD--an old bottle with new wine. Chang Gung Med J 28, 606-12

27.    Cheng ML, Ho HY, Tseng HC, Lee CH, Shih LY, Chiu DTY* (2005) Antioxidant deficit and enhanced susceptibility to oxidative damage in individuals with different forms of alpha-thalassaemia. Brit J Haematol 128:119-27 (SCI)