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Ching-Ping Tseng

Ching-Ping Tseng

Highest Degree

Ph. D. in Human Cancer Biology University

 of Wisconsin-Madison

 

 

 


 

Areas of Specialty

Cancer Biology, Platelet Biology Molecular Diagnostics, Molecular and Cellular  Biology

Office Phone

5202

Lab phone

5216

Research website:

https://sites.google.com/site/ctsenglab/

E-mail

ctsengmail.cgu.edu.tw

Lab & Research Interest

Molecular analysis of the interplay between tumor microenvironment and cancer progression

Cancer is one of the major causes of death worldwide and has a significant impact on national health. During cancer progression, tumor cells are confronted with platelet and various plasma proteins in the bloodstream that has been defined as the third microenvironment in addition to the primary tumor site (the primary tumor microenvironment) and the distant metastatic organs (the second tumor microenvironment). Experimental and clinical data all indicate that tumor cell interacting with platelets and the clotting system are important elements of tumor progression and cancer cells metastasis leading to the development of advanced stage cancer. Abrogation of cancer cell-platelet interaction thereby provides an attractive target for development of innovative cancer therapeutic regimens. We aim to identify novel regulators that are crucial for cancer cell-platelet interactions using various molecular and cellular approaches. A number of in vivo and in vitro study models have been established that can be applied for target gene discovery and analysis. Several regulators that mediate cancer cell-platelet interactions are candidates for translating to clinical application and are under intensive investigation.

 

Molecular analysis of platelet function and platelet biogenesis

Platelet is important in various physiological and pathological conditions, including hemostasis, thrombosis, and cancer. Understanding the mechanistic insight of platelet biogenesis and signaling can contribute to the development of effective therapy for thrombosis, thrombocytopenia, and cancer. Although it has been known for years that each megakarycoyte produces about 5000 platelets through the formation of proplatelet with thin tread structure protruding from the megakaryocytes, the key regulators in the control of megakaryocytic differentiation and platelet biogenesis are not yet completely understood. At present, the murine embryonic stem cells-OP9 coculture system and the lineage-specific DAB2 knockout mice are used as the study model to elucidate the functional role of the adaptor protein Disabled-2 (DAB2) in the regulation of megakaryocytic differentiation, proplatelet formation and platelet function. We expect to gain new insight on the mechanisms underlying megakaryocytic maturation and platelet biogenesis that should provide information on how functional platelets are produced in physiological condition and how to obtain and maintain appropriate platelet count under pathological condition.

Publication

1.     Chen DP, Tseng C-P (Co-first author), Wang WT, Sun CF. Genetic and mechanistic evaluation for the mixed-field agglutination in B3 blood type with IVS3+5G>A ABO gene mutation. PLoS One 7:e37272, 2012. (SCI)

2.     Hung WS, Huang CL, Fan JT, Huang DY, Yeh CF, Cheng JC, Tseng C-P (Corresponding author). The endocytic adaptor protein Disabled-2 is required for cellular uptake of fibrinogen. Biochim Biophys Acta. 1823:1778-1788, 2012. (SCI)

3.     Lee TH, Wu TS, Tseng C-P (Co-corresponding author), Qiu JT. High-resolution melting molecular signatures for rapid identification of human papillomavirus genotypes. PLoS One, 7:e42051, 2012. (SCI)

4.     Huang SB, Wu MH, Lin YH, Hsieh CH, Yang CL, Lin HC, Tseng C-P (Co-corresponding author), Lee GB. High-purity and label-free isolation of circulating tumor cells (CTCs) in a microfluidic platform by using optically-induced-dielectrophoretic (ODEP) force. Lab Chip, 13:1371-83, 2013. (SCI)

5.     Huang JT, Tseng C-P, Liao MH, Lu SC, Yeh WZ, Sakamoto N, Chen CM, Cheng JC. Hepatitis C virus replication is modulated by the interaction of non-structural protein NS5B and fatty acid synthase. J Virol. 87:4994-5004, 2013. (SCI)

6.     Lin HC, Hsu HC, Hsieh CH, Wang HM, Huang CY, Wu MH, Tseng C-P (Corresponding author). A negative selection system PowerMag for effective leukocyte depletion and enhanced detection of EpCAM positive and negative circulating tumor cells. Clin Chim Acta. 419C:77-84, 2013. (SCI)

7.     Chang SS, Hsieh WH, Liu TS, Lee SH, Wang CH, Chou HC, Yeo YH, Tseng C-P (Co-corresponding author), Lee CC. Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis - a systemic review and meta-analysis. PLoS One. 8:e62323, 2013. (SCI)

8.     Chu HC, Lee HY, Huang YS, Tseng WL, Yen CJ, Cheng JC, Tseng C-P (Corresponding author). Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice. FEBS Lett. 588:58-64, 2014. (SCI)

9.     Chen DP, Sun CF, Ning HC, Peng CT, Wang WT, Tseng C-P (Corresponding author). Real-time amplification of glyceraldehyde-3-phosphate dehydrogenase gene for quality control of leukopoor platelets. Transfusion. 54:445-50, 2014. (SCI)

10.  Chu HC, Tseng WL, Lee HY, Cheng JC, Chang SS, Yung BY, Tseng C-P (Corresponding author). Distinct effects of Disabled-2 on transferrin uptake in different cell types and culture conditions. Cell Biol Int. 38:1252-1259, 2014. (SCI)

11.  Tsai HJ, Huang CL, Chang YW, Huang DY, Lin CC, Cooper JA, Cheng JC, Tseng C-P (Corresponding author). Disabled-2 is required for efficient haemostasis and platelet activation by thrombin in mice. Arterioscler Thromb Vasc Biol 34:2404-2412, 2014. (SCI)

12.  Tseng WL, Chen TH, Huang CC, Huang YH, Yeh CF, Tsai HJ, Lee HY, Kao CY, Lin SW, Liao HR, Cheng JC, Tseng C-P (Corresponding author). Impaired thrombin generation in Reelin-deficient mice: a potential role of plasma Reelin in hemostasis. J Thromb Haemost. 12:2054-2064, 2014. (SCI)

13.  Chen DP, Sun CF, Ning HC, Peng CT, Wang WT, Tseng C-P (Corresponding author). Genetic and mechanistic evaluation for the weak A phenotype in Ael blood type with IVS6 + 5G>A ABO gene mutation. Vox Sang. 108:64-71, 2015. (SCI)

14.  Hsieh CH, Lin HC, Huang CY, Hsu HL, Wu MH, Lee CL, Chen MC, Wang HM, Tseng C-P (Corresponding author). Prognostic value of circulating tumor cells with podoplanin expression in patients with locally advanced or metastatic head and neck squamous cell carcinoma. Head Neck 37:1448-1455, 2015. (SCI)

15.  Chang YW, Hsieh PW, Chang YT, Lu MH, Huang TF, Chong KY, Liao HR, Cheng JC, Tseng C-P (Corresponding author). Identification of a novel platelet antagonist that binds to CLEC-2 and suppresses podoplanin-induced platelet aggregation and cancer metastasis. Oncotarget 6:42733-42748, 2015. (SCI)

16.  Chen DP, Tseng C-P, Lin CJ, Wang WT, Sun CF. Mechanistic evaluation for mixed-field agglutination in the K562 cell study model with exon 3 deletion of A1 gene. Ann Clin Lab Sci. 45:674-679, 2015. (SCI)

17.  Lin HC, Liou MJ, Hsu HL, Hsieh CH, Chen YA, Tseng C-P (Corresponding author), Lin JD. Combined analysis of circulating epithelial cells and serum thyroglobulin for distinguishing disease status of the patients with papillary thyroid carcinoma. Oncotarget 7: 17242-17253, 2016. (SCI)

18.  Hsieh CH, Lin HC, Huang SB, Hsueh C, Hsu HL, Wang HM, Wu MH, Tseng C-P (Corresponding author), Lin JD. Circulating epithelial cell enumeration facilitates the identification and follow-up of a patient with early stage papillary thyroid microcarcinoma: A case report. Clin Chim Acta. 454:107-111, 2016. (SCI)

19.  Chen DP, Sun CF, Ning HC, Wang WT, Tseng C-P (Corresponding author). Comprehensive analysis of RHD splicing transcripts reveals the molecular basis for the weak anti-D reactivity of Del -red blood cells. Transfus Med. 26:123-129, 2016. (SCI)

20.  Hung WS, Ling P, Cheng JC, Chang SS, Tseng C-P (Corresponding author). Disabled-2 is a negative immune regulator of lipopolysaccharide-stimulated Toll-like receptor 4 internalization and signaling. Sci Rep. 6:35343, 2016. (SCI)

21.  Chen DP, Chang SW, Jaing TH, Tseng C-P, Chen SH, Wang WT. Effect of HLA mismatching at HLA-A, -B, and -DRB1 for umbilical-cord blood transplantation in Taiwan. Clin Chim Acta. 462:162-165, 2016. (SCI)

22.  Tsai HJ, Tseng C-P (Corresponding author). The adaptor protein Disabled-2: new insights into platelet biology and integrin signaling. Thromb J. 14(Suppl 1):28, 2016. Review

23.  Cheng JC, Tseng C-P (Co-first author), Liao MH, Peng CY, Yu JS, Chuang PH, Huang JT, Chen JJW. Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes. Sci Rep. 7:4448, 2017. (SCI)

24.  Tsai HJ, Chien KY, Liao HR, Shih MS, Lin YC, Chang YW, Cheng JC, Tseng C-P (Corresponding author). Functional links between Disabled-2 Ser723 phosphorylation and thrombin signaling in human platelets. J Thromb Haemost. 15:2029-2044, 2017. (SCI)

25.  Chen DP, Wen YH, Lu JJ, Tseng C-P, Chen WL, Chang SW. Human platelet antigens are associated with febrile non-hemolytic transfusion reactions. Clin Chim Acta. 474:120-123, 2017. (SCI)

26.  Tseng C-P (Corresponding author), Leong KK, Liou MJ, Hsu HL, Lin HC, Chen YA, Lin JD. Circulating epithelial cell counts for monitoring the therapeutic outcome of patients with papillary thyroid carcinoma. Oncotarget. 8:77453-77464, 2017. (SCI)

27.  Li YR, Tseng C-P (Co-first author), Hsu HL, Lin HC, Chen YA, Chen ST, Liou MJ, Lin JD. Circulating epithelial cells as potential biomarkers for detection of recurrence in patients of papillary thyroid carcinoma with positive serum anti-thyroglobulin antibody. Clin Chim Acta. 477:74-80, 2018. (SCI)

28.  Chang YW, Tseng CP (Co-first autor), Lee CH, Hwang TL, Chen YL, Su MT, Chong KY, Lan YW, Wu CC, Chen KJ, Lu FH, Liao HR, Hsueh C, Hsieh PW. β-Nitrostyrene derivatives attenuate LPS-mediated acute lung injury via the inhibition of neutrophil-platelet interactions and NET release. Am J Physiol Lung Cell Mol Physiol. 314:L654-L669, 2018. (SCI)

29.  Chen DP, Wen YH, Lu JJ, Tseng C-P, Wang WT. Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction. J Formos Med Assoc. 2018. (SCI)

30.  Chen DP, Chang SW, Jaing TH, Wang WT, Hus FP, Tseng C-P (Corresponding author). Single nucleotide polymorphisms within HLA region are associated with disease relapse for patients with unrelated cord blood transplantation. Peer J. 6:e5228, 2018. (SCI)

31.  Lin JD, Liou MJ, Hsu HL, Leong KK, Chen YT, Wang YR, Hung WS, Lee HY, Tsai HJ, Tseng C-P (Corresponding author). Circulating epithelial cell characterization and correlation with remission and survival in patients with thyroid cancer. Thyroid. 28:1479-1489, 2018. (SCI)