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吳治慶副教授

 (Chih-Ching Wu)

學歷

長庚大學生物醫學研究所博士

任課科目

生物化學、臨床生物化學

研究專長

蛋白質體學、系統生物學、腫瘤生物學

辦公室分機

5093

實驗室分機

3562

個人網頁

臨床蛋白質體學研究室

E-mail

luckywu@mail.cgu.edu.tw

研究方向及研究室特色(Lab & Research Interest)

我們臨床蛋白質體實驗室主要以癌症研究為主。在台灣癌症是十大死因之首,每年超過80000人因其而犧牲,因此癌症的相關研究顯得格外重要。肺癌在台灣是導致死亡率最高的癌症(17.5%),每年約占新診斷出癌症的12.4%;口腔癌在台灣則是致死率第四高的癌症,每年超過2100人因其而犧牲。僅管過去的二十年中,肺癌與口腔癌的治療已有明顯的進步與改善,但是這些癌症病患的存活率卻不見明顯的增加,究其原因,在於病人診斷出時通常已為癌症晚期,因此治癒率甚差。以肺癌為例:五年存活率整體來說低於15%。因此若有有效的腫瘤標誌以早期診斷肺癌與口腔癌,定能改善癌症病患的治療與預後。我們實驗室致力於找尋肺癌與口腔癌的新穎腫瘤標誌,更進一步探討這些腫瘤標誌在腫瘤細胞中扮演的生物學意義。

我們實驗室主要以新穎的蛋白質體學(proteomics)技術,解析癌症的相關議題。細胞中執行生物功能的巨分子多為蛋白質,因此蛋白質的功能探討在癌症研究中是重要的一環。現今生物質譜儀(mass spectrometry)的發展,讓研究學者得以體學的角度研究蛋白質;例如:系統的觀察細胞中所有蛋白質的表現量、與巨分子的交互作用、位置與轉譯後修飾。因蛋白質體技術的優勢,此技術已被廣泛的運用於疾病之研究,特別是在體液的腫瘤生物標誌搜尋和鑑定上。我們實驗室致力於使用與發展蛋白質體學技術,以找尋新穎的疾病蛋白標誌,更以蛋白質體學技術探討這些蛋白在疾病中扮演的角色。

 

論文與著作(Publication)

最近五年所發表論文

  1. Chen CY, Wu CC, Huang YC, Hung CF, Wang LJ. Gender differences in the relationships among neurosteroid serum levels, cognitive function, and quality of life. Neuropsychiatr Dis Treat 2018, 14, 2389-99.
  2. Wang LJ, Lin PY, Lee Y, Huang YC, Wu CC, Hsu ST, Chen CC, Chong MY, Lin CH, Hung CF. Increased serum levels of cysteine in patients with schizophrenia: A potential marker of cognitive function preservation. Schizophr Res 2018, 192, 391-7.
  3. Lin YH, Wu CC, Peng YS, Wu CW, Chang YT, Chang KP. Detection of anti-p53 autoantibodies in saliva using microfluidic chips for the rapid screening of oral cancer. RSC Advances 2018, 8(28), 15513-21.
  4. Lin MH, Li CC, Shu JC, Chu HW, Liu CC, Wu CC. Exoproteome profiling reveals the involvement of the foldase PrsA in the cell surface properties and pathogenesis of Staphylococcus aureus. Proteomics 2018, 18(5-6), e1700195.
  5. Li HP, Peng CC, Wu CC, Chen CH, Shih MJ, Huang MY, Lai YR, Chen YL, Chen TW, Tang P and others. Inactivation of the tight junction gene CLDN11 by aberrant hypermethylation modulates tubulins polymerization and promotes cell migration in nasopharyngeal carcinoma. J Exp Clin Cancer Res 2018, 37(1), 102.
  6. Kuo RL, Chen CJ, Tam EH, Huang CG, Li LH, Li ZH, Su PC, Liu HP, Wu CC. Interactome analysis of NS1 protein encoded by influenza A H7N9 virus reveals an inhibitory role of NS1 in host mRNA maturation. J Proteome Res 2018, 17(4), 1474-84.
  7. Huang YC, Lin PY, Lee Y, Hung CF, Hsu ST, Wu CC*, Wang LJ*. Serum levels of beta-hydroxybutyrate and pyruvate, metabolic changes and cognitive function in patients with schizophrenia during antipsychotic treatment: a preliminary study. Neuropsychiatr Dis Treat 2018, 14, 799-808. (* correspondence author)
  8. Chu CS, Li DJ, Chu CL, Wu CC, Lu T. Decreased IL-1ra and NCAM-1/CD56 serum levels in unmedicated patients with schizophrenia before and after antipsychotic treatment. Psychiatry Investig 2018, 15(7), 727-32.
  9. Chu CS, Chu CL, Wu CC, Lu T. Serum nerve growth factor beta, brain- and glial-derived neurotrophic factor levels and psychopathology in unmedicated patients with schizophrenia. J Chin Med Assoc 2018, 81(6), 577-81.
  10. Wu KA*, Wu CC*, Chen CD, Chu CM, Shih LJ, Liu YC, Wang CL, Lin HH, Yang CY. Proteome profiling reveals novel biomarkers to identify complicated parapneumonic effusions. Sci Rep 2017, 7(1), 4026. (* equal contribution)
  11. Lee SY, Wang LJ, Chang CH, Wu CC, Chen HL, Lin SH, Chu CL, Lu T, Lu RB. Serum DHEA-S concentration correlates with clinical symptoms and neurocognitive function in patients with bipolar II disorder: A case-controlled study. Prog Neuropsychopharmacol Biol Psychiatry 2017, 74, 31-5.
  12. Kuo IC, Kao HK, Huang Y, Wang CI, Yi JS, Liang Y, Liao CT, Yen TC, Wu CC*, Chang KP*. Endoplasmic reticulum aminopeptidase 2 involvement in metastasis of oral cavity squamous cell carcinoma discovered by proteome profiling of primary cancer cells. Oncotarget 2017, 8(37), 61698-708. (* correspondence author)
  13. Huang YC, Hung CF, Lin PY, Lee Y, Wu CC, Hsu ST, Chen CC, Chong MY, Lin CH, Wang LJ. Gender differences in susceptibility to schizophrenia: Potential implication of neurosteroids. Psychoneuroendocrinology 2017, 84, 87-93.
  14. Chen YT, Chen HW, Wu CF, Chu LJ, Chiang WF, Wu CC, Yu JS, Tsai CH, Liang KH, Chang YS and others. Development of a multiplexed liquid chromatography multiple-reaction-monitoring mass spectrometry (LC-MRM/MS) method for evaluation of salivary proteins as oral cancer biomarkers. Mol Cell Proteomics 2017, 16(5), 799-811.
  15. Chen TW, Lee CC, Liu H, Wu CS, Pickering CR, Huang PJ, Wang J, Chang IY, Yeh YM, Chen CD and others. APOBEC3A is an oral cancer prognostic biomarker in Taiwanese carriers of an APOBEC deletion polymorphism. Nat Commun 2017, 8(1), 465.
  16. Chang CK, Chen CJ, Wu CC, Chen SW, Shih SR, Kuo RL. Cellular hnRNP A2/B1 interacts with the NP of influenza A virus and impacts viral replication. PLoS One 2017, 12(11), e0188214.
  17. Yu JS, Chen YT, Chiang WF, Hsiao YC, Chu LJ, See LC, Wu CS, Tu HT, Chen HW, Chen CC and others. Saliva protein biomarkers to detect oral squamous cell carcinoma in a high-risk population in Taiwan. Proc Natl Acad Sci USA 2016, 113(41), 11549-54.
  18. Lin YM*, Wu CC*, Chang YC, Wu CH, Ho HL, Hu JW, Chang RC, Wang CT, Ouyang P. Target disruption of ribosomal protein pNO40 accelerates aging and impairs osteogenic differentiation of mesenchymal stem cells. Biochem Biophys Res Commun 2016, 469(4), 903-10. (* equal contribution)
  19. Kuo RL, Li ZH, Li LH, Lee KM, Tam EH, Liu HM, Liu HP, Shih SR, Wu CC. Interactome analysis of the NS1 protein encoded by influenza A H1N1 virus reveals a positive regulatory role of host protein PRP19 in viral replication. J Proteome Res 2016, 15(5), 1639-48.
  20. Huang YC, Lin PY, Lee Y, Wu CC, Hsu ST, Hung CF, Chen CC, Chong MY, Lin CH, Wang LJ. beta-hydroxybutyrate, pyruvate and metabolic profiles in patients with schizophrenia: A case control study. Psychoneuroendocrinology 2016, 73, 1-8.
  21. Huang HI, Chang YY, Lin JY, Kuo RL, Liu HP, Shih SR, Wu CC. Interactome analysis of the EV71 5' untranslated region in differentiated neuronal cells SH-SY5Y and regulatory role of FBP3 in viral replication. Proteomics 2016, 16(17), 2351-62.
  22. Hsu CH, Hsu CW, Hsueh C, Wang CL, Wu YC, Wu CC, Liu CC, Yu JS, Chang YS, Yu CJ. Identification and characterization of potential biomarkers by quantitative tissue proteomics of primary lung adenocarcinoma. Mol Cell Proteomics 2016, 15(7), 2396-410.
  23. Chiang CH*, Wu CC*, Lee LY, Li YC, Liu HP, Hsu CW, Lu YC, Chang JT, Cheng AJ. Proteomics analysis reveals involvement of Krt17 in areca nut-induced oral carcinogenesis. J Proteome Res 2016, 15(9), 2981-97. (* equal contribution)
  24. Wu CC, Chu HW, Hsu CW, Chang KP, Liu HP. Saliva proteome profiling reveals potential salivary biomarkers for detection of oral cavity squamous cell carcinoma. Proteomics 2015, 15(19), 3394-404.
  25. Liu PJ, Chen CD, Wang CL, Wu YC, Hsu CW, Lee CW, Huang LH, Yu JS, Chang YS, Wu CC and others. In-depth proteomic analysis of six types of exudative pleural effusions for nonsmall cell lung cancer biomarker discovery. Mol Cell Proteomics 2015, 14(4), 917-32.
  26. Kuo RL, Lin YH, Wang RY, Hsu CW, Chiu YT, Huang HI, Kao LT, Yu JS, Shih SR, Wu CC. Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication. J Proteome Res 2015, 14(4), 1818-30.
  27. Ke WJ, Hsueh YH, Cheng YC, Wu CC, Liu ST. Water surface tension modulates the swarming mechanics of Bacillus subtilis. Front Microbiol 2015, 6, 1017.
  28. Chen CL, Chung T, Wu CC, Ng KF, Yu JS, Tsai CH, Chang YS, Liang Y, Tsui KH, Chen YT. Comparative tissue proteomics of microdissected specimens reveals novel candidate biomarkers of bladder cancer. Mol Cell Proteomics 2015, 14(9), 2466-78.
  29. Yeh YM, Chen CY, Huang PR, Hsu CW, Wu CC*, Wang TC*. Proteomic analyses of genes regulated by heterogeneous nuclear ribonucleoproteins A/B in Jurkat cells. Proteomics 2014, 14(11), 1357-66. (* correspondence author)
  30. Wu CC, Chang YT, Chang KP, Liu YL, Liu HP, Lee IL, Yu JS, Chiang WF. Salivary auto-antibodies as noninvasive diagnostic markers of oral cavity squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev 2014, 23(8), 1569-78.
  31. Wang LJ, Wu CC, Lee SY, Tsai YF. Salivary neurosteroid levels and behavioural profiles of children with attention-deficit/hyperactivity disorder during six months of methylphenidate treatment. J Child Adolesc Psychopharmacol 2014, 24(6), 336-40.
  32. Wang LJ, Huang HY, Huang MP, Liou W, Chang YT, Wu CC, Ojcius DM, Chang YS. The microtubule-associated protein EB1 links AIM2 inflammasomes with autophagy-dependent secretion. J Biol Chem 2014, 289(42), 29322-33.
  33. Hsu CW, Yu JS, Peng PH, Liu SC, Chang YS, Chang KP, Wu CC. Secretome profiling of primary cells reveals that THBS2 is a salivary biomarker of oral cavity squamous cell carcinoma. J Proteome Res 2014, 13(11), 4796-807.
  34. Chen CD, Wang CL, Yu CJ, Chien KY, Chen YT, Chen MC, Chang YS, Wu CC*, Yu JS*. Targeted proteomics pipeline reveals potential biomarkers for the diagnosis of metastatic lung cancer in pleural effusion. J Proteome Res 2014, 13(6), 2818-29. (* correspondence author)
  35. Chen CC, Liu HP, Chao M, Liang Y, Tsang NM, Huang HY, Wu CC, Chang YS. NF-kappaB-mediated transcriptional upregulation of TNFAIP2 by the Epstein-Barr virus oncoprotein, LMP1, promotes cell motility in nasopharyngeal carcinoma. Oncogene 2014, 33(28), 3648-59.

專利

1.  余兆松、張雅婷、吳治慶、石宜銘、張玉生。胰臟癌之血清生物檢測標誌及其應用(A serological marker for detecting pancreatic cancer and a method for using the serological marker)。中華民國專利發明第I408370號,專利權期間: 2013/9/11~2031/5/18

專書論文

  1. Wu CC, Chang YS, Chow KP. The immunogenicity of tumor cells that express Epstein-Barr virus encoded LMP1 isolated from NPC in immunocompetent mice. The 10th international congress of immunology. 1998, 1257-1261, India.
  2. Chen YT, Parker CE, Chen HW, Chen CL, Domanski D, Smith DS, Wu CC, Chung T, Liang KH, Chen MC, Chang YS, Borchers CH, Yu JS. Chapter 10: A Pipeline that Integrates the Discovery and Verification Studies of Urinary Protein Biomarkers Reveals Candidate Markers for Bladder Cancer in Comprehensive Biomarker Discovery and Validation for Clinical Application. Peter Horvatovich, ‎Rainer Bischoff, ‎David E. Thurston (Editors), Royal Society of Chemistry (RSC), 2013.