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郭 瑞 琳 (Rei-Lin Kuo)






分子病毒學 病毒與細胞交互作用








研究方向及研究室特色(Lab & Research Interest)

1.     The induction of the IFN expression is a critical event for innate immunity to disrupt viral replication in virus-infected cells. It has been demonstrated that viruses can evade host innate immunity by targeting the pathway for type I IFN activation. The regulation of the type I IFN production has been considered an important factor for virus pathogenicity. Since EV71 is an important human pathogen that periodically causes several outbreaks with severe neurological complications in recent years in Taiwan, our lab is endeavoring to elucidate the mechanism for the regulation of signaling pathway for IFN-b production in EV71-infected cells. The understanding of the regulation of IFN production may provide the information for EV71 pathogenicity and the basis for the development of new antiviral drugs.

2.     The viral RNA synthesis is directly involved in the virus replication during influenza A virus infection. Base on pathogenicity studies, the viral polymerase has been considered as one of the contributors for the virulence of influenza viruses. Since the in vitro system has been established for studying the replication and transcription of influenza polymerase, our lab is interested in verifying the polymerase activities of highly pathogenic influenza virus strains by using the established in vitro system.


 Publications (Since 2008):

1.        Rei-Lin Kuo, Shu-Li Yang, Yi-Chun Liu, Lu-Ting Chen, Chee-Keng Mok, Shu-Ming Kuo, Shin-Ru Shih, and Kuo-Chien Tsao. Influenza A/B virus detection and influenza A virus subtyping with emphasis on the novel H7N9 virus by using multiplex real-time RT-PCR. 2014. Journal of Virological Methods, 208:41-46

2.        Yen-Chin Liu, Rei-Lin Kuo, Jing-Yi Lin, Peng-Nien Huang, Yi Huang, Hsuan Liu, Jamine J. Arnold, Shu-Jen Chen, Robert Yung-Liang Wang, Craig E. Cameron, Shin-Ru Shih. Cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with Prp8. 2014. PLoS Pathogens, 10(6):e1004199

3.        Chih-Heng Huang, Chi-Jene Chen, Chia-Tsui Yen, Cheng-Ping Yu, Peng-Nien Huang, Rei-Lin Kuo, Sue-Jane Lin, Cheng-Kai Chang, and Shin-Ru Shih. Caspase-1 deficient mice are more susceptible to influenza A virus infection with PA variation. 2013. Journal of Infectious Diseases, 208(11):1898-905

4.        Robert Y.L. Wang, Rei-Lin Kuo, Wei-Chieh Ma, Hsing-I Huang, Jau-Song Yu, Sih-Min Ye, Chi-Ruei Huang, Shin-Ru Shih. Heat shock protein-90-beta facilitates enterovirus 71 viral particles assembly. 2013. Virology, 443(2):236-247

5.        Rei-Lin Kuo*, Li-Ting Kao, Sue-Jane Lin, Robert YL. Wang, and Shin-Ru Shih. MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. 2013. PLoS One, 8(5):e63431. (*corresponding author)

6.        Rei-Lin Kuo and Shin-Ru Shih. Strategies to develop antivirals against enterovirus 71. 2013. Virology Journal, 10:28. (Highly accessed article)

7.        Qiaozhen Ye, Tom S. Y. Guu, Douglas A. Mata, Rei-Lin Kuo, Bartram Smith, Robert M. Krug, and Yizhi J. Tao. Biochemical and structural Evidence in support of a coherent model for the formation of the double-helical influenza A virus ribonucleoprotein. 2013. mBio, 4(1):e00467-12

8.        Rei-Lin Kuo, Chen Zhao, Meghana Malur, and Robert M. Krug. The NS1 proteins of different influenza A virus subtypes employ strikingly different strategies counter the host antiviral response. 2010. Virology, 408(2):146-158

9.        Chen Zhao, Tien-Ying Hsiang, Rei-Lin Kuo, and Robert M. Krug. ISG15 conjugation system targets the viral NS1 protein in influenza A virus-infected cells. 2010. Proceedings of the National Academy of Sciences of the USA, 107(5), 2253-2258

10.     Chen Zhao, Rei-Lin Kuo, Robert M. Krug. Chapter 1: The NS1 Protein of Influenza A Virus. 2010. Influenza: Molecular Virology. Caister Academic Press. Publication date: February 2010

11.     Rei-Lin Kuo and Robert M. Krug. 2009. The influenza virus polymerase is an integral component of the NS1A:CPSF30 complex in infected cells. Journal of Virology, 83:1611-1616

12.     Laura L. Newcomb*, Rei-Lin Kuo*, Qiaozhen Ye, Yizhi Jane Tao, and Robert M. Krug. 2009. Interaction of the influenza A virus nucleocapsid protein with the viral RNA polymerase potentiates unprimed viral RNA replication. Journal of Virology, 83:29-36 (*equal contribution)

13.     Kalyan Das, Li-Chung Ma, Rong Xiao, Brian Radvansky, James Aramini, Li, Zhao, Jesper Marklund, Rei-Lin Kuo, Karen Y. Twu, Eddy Arnold, Robert M. Krug, Gaetano T. Montelione. 2008. Structural basis for suppression by influenza A virus of a host antiviral response. Proceedings of the National Academy of Sciences of the USA, 105:13093-13098

Conference presentations (Since 2008):

1.       Kuo, RL., Yang, SL., Liu, YC., Chen, LT., Mok, CK., Kuo SM., Shih, SR., and Tsao, KC. Differential Detection of Avian H7N9 Influenza A Virus Using Highly Sensitive Multiplex Real-time RT-PCR. American Society for Virology 33rd Annual Meeting. June 21-25, 2014, Fort Collins, Colorado, USA

2.       Kuo, RL., Kao, LT., Lin, SJ., Wang RY., and Shih, SR. MDA5 plays an important role in enterovirus 71 RNA-mediated IRF3 activation. American Society for Virology 32nd Annual Meeting. July 19-24, 2013, State College, Pennsylvania, USA

3.       Kuo, RL., Hsieh, EF., Lin, LT., Chiang, C, Chang, YC., Chang CK., Chen GW., and Shih, SR. Differential roles of SLU7 on the expression of M2 ion channel protein of influenza A virus in human versus avian cells. XVth International Congress of Virology. Sept 11-16, 2011, Sapporo, Japan

4.       Kuo, RL., Zhao, C., Malur, M., and Krug, RM. Whether interferon- transcription is activated in influenza A virus-infected cells depends on the source of the viral NS1. American Society for Virology 29th Annual Meeting. July 17-21, 2010, Bozeman, Montana, USA

5.       Kuo, RL., Marklund, J., Twu, KY., and Krug, RM. The influenza A virus polymerase binds to and stabilizes the complex of the NS1A protein with CPSF30 that mediates the inhibition of IFN mRNA production. American Society for Virology 27th Annual Meeting. July 12-16, 2008, Ithaca, New York, USA