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郭瑞琳副教授

郭 瑞 琳 (Rei-Lin Kuo)

學歷

陽明大學微生物暨免疫學博士

任課科目

高等微生物及感染症學、分子生物學

研究專長

分子病毒學 病毒與細胞交互作用

辦公室分機

3775

實驗室分機

3725

個人網頁

分子病毒實驗室

E-mail

rlkuo@mail.cgu.edu.tw

研究方向及研究室特色(Lab & Research Interest)

1.     The induction of the IFN expression is a critical event for innate immunity to disrupt viral replication in virus-infected cells. It has been demonstrated that viruses can evade host innate immunity by targeting the pathway for type I IFN activation. The regulation of the type I IFN production has been considered an important factor for virus pathogenicity. Since EV71 is an important human pathogen that periodically causes several outbreaks with severe neurological complications in recent years in Taiwan, our lab is endeavoring to elucidate the mechanism for the regulation of signaling pathway for IFN-b production in EV71-infected cells. The understanding of the regulation of IFN production may provide the information for EV71 pathogenicity and the basis for the development of new antiviral drugs.

2.     The viral RNA synthesis is directly involved in the virus replication during influenza A virus infection. Base on pathogenicity studies, the viral polymerase has been considered as one of the contributors for the virulence of influenza viruses. Since the in vitro system has been established for studying the replication and transcription of influenza polymerase, our lab is interested in verifying the polymerase activities of highly pathogenic influenza virus strains by using the established in vitro system.

論文與著作(Publication)

Publications (since 2010):

  1. Rei-Lin Kuo, Chi-Jene Chen, Ee-Hong Tam, Chung-Guei Huang, Li-Hsin Li, Zong-Hua Li, Pei-Chia Su, Hao-Ping Liu, and Chih-Ching Wu. Interactome analysis of NS1 protein encoded by influenza A H7N9 virus reveals an inhibitory role of NS1 in host mRNA maturation. 2018. Journal of Proteome Research, 17: 1474-1484.
  2. Cheng-Kai Chang, Chi-Jene Chen, Chih-Ching Wu, Shiau-Wen Chen, Shin-Ru Shih*, and Rei-Lin Kuo*. Cellular hnRNP A2/B1 interacts with the NP of influenza A virus and impacts viral replication. 2017. PLoS ONE, 12(11):e0188214. (*co-corresponding author)
  3. Chi-JeneChen, Guan-HongWu, Rei-LinKuo*, Shin-Ru Shih*. Role of the intestinal microbiota in the immunomodulation of influenza virus infection. 2017. Microbes and Infection, 19:570-579. (*co-corresponding author)
  4. Yu-Hsiu Chang, Kean Seng Lau, Rei-Lin Kuo, Jim-Tong Horng. dsRNA binding domain of PKR is proteolytically released by Enterovirus A71 to facilitate viral replication. 2017. Frontiers in Cellular and Infection Microbiology, 7:284.
  5. Hsing-I Huang, Ying-Ying Chang, Jhao-Yin Lin, Rei-Lin Kuo, Hao-Ping Liu, Shin-Ru Shih, Chih-Ching Wu. Interactome analysis of the EV71 5' untranslated region in differentiated neuronal cells SH-SY5Y and regulatory role of FBP3 in viral replication. 2016. Proteomics, 16(17): 2351-62
  6. Rei-Lin Kuo,* Zong-Hua Li, Li-Hsin Li, Kuo-Ming Lee, Ee-Hong Tam, Helene M. Liu, Hao-Ping Liu, Shin-Ru Shih, and Chih-Ching Wu.* Interactome analysis of the NS1 protein Encoded by influenza A H1N1 virus reveals a positive regulatory role of host protein PRP19 in viral replication. 2016. Journal of Proteome Research, 15: 1639-1648. (*co-corresponding author)
  7. Rei-Lin Kuo,* Li-Hsin Li, Sue-Jane Lin, Zong-Hua Li, Guang-Wu Chen, Cheng-Kai Chang, Yi-Ren Wang, Ee-Hong Tam, Yu-Nong Gong, Robert M. Krug,* and Shin-Ru Shih. Role of N terminus truncated NS1 proteins of influenza A virus in inhibiting IRF3 activation. 2016. Journal of Virology, 90(9): 4696-4705. (*co-corresponding author)
  8. Kin Fong Lei, Jun-Liang Liu, Chia-Hao Huang, Rei-Lin Kuo, and Ngan-Ming Tsang. A reagent-ready-on-chip microfluidic immunoassay system for rapid detection of influenza A H1N1 and H3N2 viruses. 2016. BioChip Journal. 10(1): 34-41.
  9. Kin Fong Lei,* Chia-Hao Huang, Rei-Lin Kuo,* Cheng-Kai Chang, Kuan-Fu Chenf, Kuo-Chien Tsao, and Ngan-Ming Tsang. Paper-based enzyme-free immunoassay for rapid detection and subtyping of influenza A H1N1 and H3N2 viruses. 2015. Analytica Chimica Acta, 883:37–44. (*co-corresponding author)
  10. Rei-Lin Kuo,* Ya-Han Lin, Robert Yung-Liang Wang, Chia-Wei Hsu, Yi-Ting Chiu, Hsing-I Huang, Li-Ting Kao, Jau-Song Yu, Shin-Ru Shih, and Chih-Ching Wu.* Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication. 2015. Journal of Proteome Research, 14:1818-1830. (*co-corresponding author)
  11. Yu-Nong Gong, Guang-Wu Chen, Chi-Jene Chen, Rei-Lin Kuo, and Shin-Ru Shih. 2014. Computational Analysis and Mapping of Novel Open Reading Frames in Influenza A Viruses. PLoS ONE, 9(12): e115016.
  12. Sue-Jane Lin, Ming Lo, Rei-Lin Kuo, Shin-Ru Shih, David M Ojcius, Jean Lu, Chien-Kuo Lee, Hui-Chen Chen, Meei Yun Lin, Chuen-Miin Leu, Chia-Ni Lin, and Ching-Hwa Tsai. The pathological effects of CCR2+ inflammatory monocytes are amplified by an IFNAR1-triggered chemokine feedback loop in highly pathogenic influenza infection. 2014. Journal of Biomedical Science, 21:99.
  13. Kuo-Feng Weng, Chuan-Tien Hung, Po-Ting Hsieh, Mei-Ling Li, Guang-Wu Chen, Yu-An Kung, Peng-Nien Huang, Rei-Lin Kuo, Li-Lien Chen, Jing-Yi Lin, Robert Yung-Liang Wang, Shu-Jen Chen, Petrus Tang, Jim-Tong Horng, Hsing-I Huang, Jen-Ren Wang, David M. Ojcius, Gary Brewer, and Shin-Ru Shih. A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells. 2014. Nucleic Acids Research, 42:12789-12805.
  14. Hsing-I Huang, Jhao-Yin Lina, Hsin-Hsu Chen, Shiou-Bang Yeh, Rei-Lin Kuo, Kuo-Feng Weng, and Shin-Ru Shih. Enterovirus 71 infects brain-derived neural progenitor cells. 2014. Virology, 468-470:592-600.
  15. Rei-Lin Kuo, Shu-Li Yang, Yi-Chun Liu, Lu-Ting Chen, Chee-Keng Mok, Shu-Ming Kuo, Shin-Ru Shih, and Kuo-Chien Tsao. Influenza A/B virus detection and influenza A virus subtyping with emphasis on the novel H7N9 virus by using multiplex real-time RT-PCR. 2014. Journal of Virological Methods, 208:41-46.
  16. Yen-Chin Liu, Rei-Lin Kuo, Jing-Yi Lin, Peng-Nien Huang, Yi Huang, Hsuan Liu, Jamine J. Arnold, Shu-Jen Chen, Robert Yung-Liang Wang, Craig E. Cameron, Shin-Ru Shih. Cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering the nucleus and interfering with Prp8. 2014. PLoS Pathogens, 10(6):e1004199.
  17. Chih-Heng Huang, Chi-Jene Chen, Chia-Tsui Yen, Cheng-Ping Yu, Peng-Nien Huang, Rei-Lin Kuo, Sue-Jane Lin, Cheng-Kai Chang, and Shin-Ru Shih. Caspase-1 deficient mice are more susceptible to influenza A virus infection with PA variation. 2013. Journal of Infectious Diseases, 208(11):1898-905.
  18. Robert Y.L. Wang, Rei-Lin Kuo, Wei-Chieh Ma, Hsing-I Huang, Jau-Song Yu, Sih-Min Ye, Chi-Ruei Huang, Shin-Ru Shih. Heat shock protein-90-beta facilitates enterovirus 71 viral particles assembly. 2013. Virology, 443(2):236-247.
  19. Rei-Lin Kuo*, Li-Ting Kao, Sue-Jane Lin, Robert YL. Wang, and Shin-Ru Shih. MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. 2013. PLoS ONE, 8(5):e63431. (*corresponding author)
  20. Rei-Lin Kuo and Shin-Ru Shih. Strategies to develop antivirals against enterovirus 71. 2013. Virology Journal, 10:28. (Highly accessed article)
  21. Qiaozhen Ye, Tom S. Y. Guu, Douglas A. Mata, Rei-Lin Kuo, Bartram Smith, Robert M. Krug, and Yizhi J. Tao. Biochemical and structural Evidence in support of a coherent model for the formation of the double-helical influenza A virus ribonucleoprotein. 2013. mBio, 4(1):e00467-12.
  22. Rei-Lin Kuo, Chen Zhao, Meghana Malur, and Robert M. Krug. Influenza A virus strains that circulate in humans differ in the ability of their NS1 proteins to block the activation of IRF3 and interferon-β transcription. 2010. Virology, 408(2):146-158.
  23. Chen Zhao, Tien-Ying Hsiang, Rei-Lin Kuo, and Robert M. Krug. ISG15 conjugation system targets the viral NS1 protein in influenza A virus-infected cells. 2010. Proceedings of the National Academy of Sciences of the USA, 107(5), 2253-2258.
  24. Chen Zhao, Rei-Lin Kuo, Robert M. Krug. Chapter 1: The NS1 Protein of Influenza A Virus. 2010. Influenza: Molecular Virology. Caister Academic Press. Publication date: February 2010.

Conference presentations:

  1. Kuo RL., Tam EH., Chen CJ., Li ZH., Li LH., Su PC., and Wu CC. Systemic Identification of Host Proteins Interacting with Influenza A H7N9 NS1 protein Reveals Its Regulatory Role of NS1 in Host mRNA Maturation. American Society for Virology 37th Annual Meeting, July 14-18, 2018, College Park, Maryland, USA
  2. Kuo RL., Li ZH., Li LH., Lee KM., Tam EH., Shih SR., and Wu CC. Systemic analysis of the host proteins that interact with the NS1 protein encoded by influenza A H1N1 virus by proteomic approach. American Society for Virology 36th Annual Meeting, June 24-28, 2017, Madison, Wisconsin, USA
  3. Kuo, RL., Lin, YH., Wang, RYL., Yu, JS., Shih, SR., and Wu, CC. A proteome-wide dysregulation analysis identifies the involvement of NEDD4L and PSMF1 in EV71 replication. Keystone symposia for 2016 conference on: Positive-strand RNA viruses. May 1-5, 2016, Austin, Texas, USA
  4. Kuo, RL., Lin, YH., Wang, RYL., Yu, JS., Shih, SR., and Wu, CC. Identification of host proteins involved in EV71 replication by proteomics analysis. American Society for Virology 34th Annual Meeting. July 11-15, 2015, Western University Ontario, London, Ontario, Canada
  5. Kuo, RL., Yang, SL., Liu, YC., Chen, LT., Mok, CK., Kuo SM., Shih, SR., and Tsao, KC. Differential Detection of Avian H7N9 Influenza A Virus Using Highly Sensitive Multiplex Real-time RT-PCR. American Society for Virology 33rd Annual Meeting. June 21-25, 2014, Fort Collins, Colorado, USA
  6. Kuo, RL., Kao, LT., Lin, SJ., Wang RY., and Shih, SR. MDA5 plays an important role in enterovirus 71 RNA-mediated IRF3 activation. American Society for Virology 32nd Annual Meeting. July 19-24, 2013, State College, Pennsylvania, USA
  7. Kuo, RL., Hsieh, EF., Lin, LT., Chiang, C, Chang, YC., Chang CK., Chen GW., and Shih, SR. Differential roles of SLU7 on the expression of M2 ion channel protein of influenza A virus in human versus avian cells. XVth International Congress of Virology. Sept 11-16, 2011, Sapporo, Japan