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黃幸宜助理教授

(Hsing-I Huang)

學歷

美國紐澤西羅格斯大學微生物與分子遺傳學博士

 http://mip.cgu.edu.tw/ezfiles/35/1035/img/90/teacher16.jpg

任課科目

醫用生物技術學、醫檢概論

研究專長

腫瘤免疫學、細胞生物學

辦公室分機

3721

實驗室分機

3725

個人網頁

病毒暨幹細胞生物實驗室

E-mail

hihuang@mail.cgu.edu.tw

研究方向及研究室特色(Lab & Research Interest)

  1. 研究幹細胞在組織工程方面的應用
            幹細胞由於其自我更新分化為其它體細胞能力,因此能應用於再生醫學上以修補受損的器官或組織。而成體幹細胞由於沒有一般胚胎幹細胞的缺點如免疫排斥,腫瘤生成以及道德爭議,因此在醫療應用上更具潛力。研究發現不同種類的成體幹細胞具有不同的分化能力,因此我們希望可以將合適的幹細胞應用於特定的組織再生醫學應用上,此外亦會合併基因轉殖、RNAi等技術以發展更有效的方法。
  2. 探討病毒與宿主幹細胞之間的關係       

        ​幹細胞極容易被各種因素影響,包括化學藥劑,生長激素,表面分子等,甚至物理因素也能夠左右其分化路徑。然而目前我們對於生物因素,例如細菌或病毒感染是否能影響幹細胞的特性則較不了解。有一些研究指出病毒的感染能使得某些成體幹細胞分化為特定的體細胞,但是對於其它幹細胞特性的影響之研究則較為缺乏。因此,我們研究室目前致力於研究病毒感染對於幹細胞的影響。尤其針對一些新興病毒,是否能經由感染幹細胞而影響其致病機轉是我們相當有興趣的一個研究方向。

論文與著作(Publication)

最近五年所發表論文 

  1. Huang HI*, Chio CC, Lin JY. Inhibition of EV71 by curcumin in intestinal epithelial cells. PLoS One, 2018 Jan 25;13(1)
  2. Huang HI*, Chang YY, Lin JY, Kuo RL, Liu HP, Shih SR, Wu CC. Interactome analysis of the EV71 5' untranslated region in differentiated neuronal cells SH-SY5Y and regulatory role of FBP3 in viral replication. Proteomics, 2016, Sep. 16; (17):2351-62.
  3. Huang HI*, Shih SR. Neurotropic Enterovirus Infections in the Central Nervous System. Viruses, 2015 Nov 24;7(11):6051-66.
  4. Weng KF, Hsieg PT, Huang HI, Shih SR. Mammalian RNA virus-derived small RNA: biogenesis and functional activity. Microbes Infect. 2015 Aug;17(8):557-63.
  5. Kuo RL, Lin YH, Wang RY, Hsu CW, Chiu YT, Huang HI, Kao LT, Yu JS, Shih SR, Wu CC. Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication. J Proteome Res. 2015 Apr 3;14(4):1818-3
  6. Weng KF, Hung CT, Hsieh PT, Li ML, Chen GW, Kung YA, Huang PN, Kuo RL, Chen LL, Lin JY, Wang RY, Chen SJ, Tang P, Horng JT, Huang HI, Wang JR, Ojcius DM, Brewer G, Shih SR. A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells. Nucleic Acids Res. 2014 Nov 10;42(20):12789-805. 2. Huang HI*, Lin JY, Chen
  7. Huang HI, Lin JY, Yeh SB, Kuo RL, Weng KF, Shih SR. Enterovirus 71 infects brain-derived neural progenitor cells. Virology. 2014 Oct 6;468-470C:592-600.
  8. Huang HI*, Chen SK, Wang RY, Shen CR, Cheng YC. Human foreskin fibroblast-like stromal cells can differentiate into functional hepatocytic cells. Cell Biol Int. 2013 Dec; 37(12):1308-19.
  9. Wang RY, Kuo RL, Ma WC, Huang HI, Yu JS, Yen SM, Huang CR, Shih SR. Heat shock protein-90-beta facilitates enterovirus 71 viral particles assembly. Virology. 2013 Sep 1;443(2):236-47.
  10. Chen CC, Huang HI*, Louis CL, Lin KT, Ron Y. Isolation of multilineage progenitors from mouse brain. In Vitro Cell Dev Biol Anim. 2013 May;49(5):307-14. 

Abstracts

  1. Hsin-Hsu Chen and Hsing-I Huang. The Effects of Enterovirus 71 Infection on Mouse Neural Stem Cells. International symposium on RNA Viruses 2011.
  2.  Hsing-I Huang, Shu-Hui Chan, Tsung-Ru Wu, Jen-Der Song. Comaparative characterization of multipotent cells isolated from human amniotic membrane and placenta. ISSCR 9th Annual Meeting, Toronto, CA. 2011.
  3. The molecular mechanism of enterovirus 71 infection in the neuronal progenitor cells. Lin JY, Chan SH, Su LC, Huang HI, Shih SR. International symposium of RNA Viruses 2010.
  4. Hsing-I Huang, CC Cheng, SK Chen, Hsiao-Tun Liu. The comparison of human FDSC and PDMCs in their in vitro hepatic differentiation abilities. ISSCR 8th Annual Meeting, San Francisco, USA. 2010.
  5. In Vitro Hepatic Differentiation of Multipotent Cells Derived from Human Placenta and Umbilical Cord. Hsing-I Huang, Tsung-Hsuan Lai, B Linju Yen, Yao-Chang Chen, Shu-Hui Chan, Chih-Cheng Chien5th ISSCR Symposium, Cairns, AU. 2007.
  6. Expression of hepatocyte-like phenotypes in human placenta-derived multipotent cells after HGF and FGF-4 induction. Hsing-I Huang, Tsung-Hsuan Lai, Fa-Kung Lee, B Linju Yen, Yao-Chang Chen, Shu-Hui Chan, Chih-Cheng Chien. 國際幹細胞研討會, Taipei, Taiwan, 2006.
  7. Differential Maturation of Human Monocyte-derived Dendritic Cells by Lipopolysaccharide and poly I:C. Li-Chiu Su , and Hsing-I Huang*.台灣分子醫學研討會Taipei, Taiwan, 2006.
  8. Isolation of Multipotent Progenitors from Human Term Umbilical Cord. Shu-Hui Chan, Tsung-Hsuan Lai and Hsing-I Huang*.台灣分子醫學研討會Taipei, Taiwan, 2006.

Books

  1. Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin. Hsing-I Huang* and Chung-Zu Wu. Somatic Stem Cells: Protocol for Methods in Molecular Biology (2011), Humana/Springer press
  2. Huang HI*. Isolation of Placenta-Derived Multipotent Cells from Human Placenta and In Vitro Differentiation into Hepatocyte-Like Cells. Current Protocols in Stem Cell Biology. Unit 1H.1, Wiley Interscience.
  3. Chan MM, Huang HI, Mattiacci JA, Fong D. 2002, Jul, Modulation of cytokine gene expression by curcumin.  In “Food factors for disease prevention and health promotion” edited by Shahidi F, Ho CT, and Watanabe S. American Chemical Society Press, Washington DC.

Patents

  1. 具多分化能力的胎盤幹細胞及其製備方法。陳耀昌,顏伶汝,簡志誠,黃幸宜 (I299752)
  2. 胎盤幹細胞及其製備方法。陳耀昌,顏伶汝,簡志誠,黃幸宜 (I307717)
  3. Y.C. Chen, L.J. Yen, C.C. Chien, H.I. Huang “Multipotent Stem Cell and Methods Thereof” U.S. Patent Pending, 2004. (60/535502)