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沈家瑞副教授

沈 家 瑞 (Chia-Rui Shen)

學歷

英國布理斯托大學免疫學博士

http://mip.cgu.edu.tw/ezfiles/35/1035/img/1302/464218227.jpg

任課科目

臨床血清及免疫學、高等免疫學、應用免疫技術、流式細胞儀分折技術實驗、尖端生物技術實驗、分子影像

研究專長

免疫療法、醫學檢驗、疫苗發展、分子影像

辦公室分機

5200

實驗室分機

5200

個人網頁

病理免疫研究室

E-mail

crshen@mail.cgu.edu.tw

研究方向及研究室特色(Lab & Research Interest)

      我們的研究主題主要是在疾病的病程發展中探討免疫反應的發生與調控,進而發展特異性的免疫療法。我們的研究目標包括:

1. To determine how priming of the antigen-specific T-cells occurs.

2. To investugate and retune the regulatory defect of such T cells.

3. To develop high efficacy immunotherapy.

      除了致力於了解免疫反應參與或調控致病機制外,我們也藉由檢測免疫反應相關分子來幫助疾病診斷,做為監測疾病惡化或治療療效的評估準則。例如利用ELISA, Flow cytometry, real-time RT-PCR來檢測細胞激素、免疫細胞族群或其他基因分子表現等(目前著重在自體免疫疾病及癌症病人的血液檢體)。在深入了解免疫反應的發生與調控上,我們也利用病毒及非病毒載體送入免疫分子,來探究其對免疫反應之影響,以發展更有效的疫苗及免疫療法對抗過敏或癌症等疾病。

論文與著作(Publication)

Journal Articles

  1. Perry FE, Barker RN, Mazza G., Day, MJ, Wells AD, Shen CR, Schofield, AE, Elson CJ Autoreactive T cell specificity in autoimmune haemolytic anaemia of the NZB mouse. Eur. J. Immunol. 1996 Jan; 26(1):136-41. SCI=4.865 (18.18%).

  1. Shen CR, Mazza G., Perry FE, Beech JT, Thompson SJ, Corato A, Newton S, Barker RN, Elson CJ. T-helper 1 dominated responses to erythrocyte Band 3 in NZB mice. Immunology 1996 Oct; 89(2):195-9. SCI=3.432 (32.23%)

  2. Barker RN, Shen CR, Mazza G., Perry FE, Hall AM, Elson CJ. T-cell subsets in autoimmune haemolytic anaemia. Biochem. Soc. Trans. 1997 May; 25(2):312S. SCI=1.907 (42.39%)

  3. Corato A, Shen CR, Mazza G., Barker RN, Day MJ. Proliferative responses of peripheral blood mononuclear cells from normal dogs and dogs with autoimmune haemolytic anaemia to red blood cell antigens. Vet. Immunol Immunopathol. 1997 Nov; 59(3-4):191-204. SCI=2.979 (8.96%)

  4. Shen CR, Wraith DC, Elson CJ. Splenic but not thymic autoreactive T-cells from New Zealand Black mice respond to a dominant erythrocyte Band 3 peptide. Immunology. 1999 Apr; 96(4):595-9. SCI=3.432 (32.23%)

  5. Shen CR, Ward FJ, Devine A, Luross JA, Lowrey PA, Wraith DC, Elson CJ, Barker RN. Characterization of the dominant autoreactive T-cell epitope in spontaneous autoimmune haemolytic anaemia of the NZB mouse. J. Autoimmun. 2002 Mar; 18(2):149-57. SCI=7.881 (10.73%)

  6. Barker RN, Shen CR, Elson CJ. T-cell specificity in murine autoimmune haemolytic anaemia induced by rat red blood cells. Clin. Exp. Immunol. 2002 Aug; 129(2):208-13. SCI=2,853 (46.28%)

  7. Shen CR (correspondence), Chiu DTY. Initiation with PBL in the Reform of Medical Technology Education in Taiwan. PBL in Action 2003 Apr; 1: 2-3.

  8. Lin MH, Chiu DTY, Shih SR, Chou YP, Shen WS, Shen CR (correspondence). Performance of PBL in Medical Technology Education. PBL in Action 2003 Apr; 1: 5-6.

  9. Shen CR, Youssef AR, Devine A, Bowie L, Hall AM, Wraith DC, Elson CJ, Barker RN. Peptides containing a dominant T-cell epitope from red cell band 3 have in vivo immunomodulatory properties in NZB mice with autoimmune hemolytic anemia. Blood 2003 Nov 15; 102(10):3800-6. Epub 2003 Jun 26. SCI=10.432 (3.23%)

  10. Youssef AR, Shen CR, Lin CL, Barker RN, Elson CJ. IL-4 and IL-10 modulate autoimmune haemolytic anaemia in NZB mice. Clin. Exp. Immunol. 2005 Jan; 139(1):84-9. SCI=2.853 (46.28%)

  11. Liu SJ, Tsai JP, Shen CR, Sher YP, Hsieh CL, Yeh YC, Chou AH, Chang SR, Hsiao KN, Yu FW, Chen HW. Induction of a distinct CD8 Tnc17 subset by transforming growth factor-beta and interleukin-6. J. Leukoc. Biol. 2007 Aug; 82(2):354-60. Epub 2007 May 15. SCI=4.605 (19.01%)

  12. Hall AM, Ward FJ, Shen CR (equal to first), Rowe C, Bowie L, Devine A, Urbaniak SJ, Elson CJ, Barker RN. Deletion of the dominant autoantigen in NZB mice with autoimmune hemolytic anemia: effects on autoantibody and T-helper responses. Blood 2007 Dec 15; 110(13):4511-7. Epub 2007 Sep 4. SCI=10.432 (3.23%)

  13. Chiu CC, Lin CY, Lee LY, Chen YJ, Kuo TF, Chang JT, Liao CT, Wang HM, Yen TC, Shen CR, Liao SK, Cheng AJ. Glucose-regulated protein 78 regulates multiple malignant phenotypes in head and neck cancer and may serve as a molecular target of therapeutic intervention. Mol. Cancer Ther. 2008 Sep; 7(9):2788-97. SCI=5.003 (17.02)

  14. Susanto J, Lin YH, Chen YN, Shen CR, Yan YT, Tsai ST, Chen CH, Shen CN. Porphyrin homeostasis maintained by ABCG2 regulates self-renewal of embryonic stem cells. PLoS ONE 2008 Dec; 3(12):e4023. Epub 2008 Dec 24.

  15. Liu HL, Hsu PH, Chu PC, Wai YY, Chen JC, Shen CR, Yen TC, Wang JJ. Magnetic resonance imaging enhanced by superparamagnetic iron oxide particles: usefulness for distinguishing between focused ultrasound-induced blood-brain barrier disruption and brain hemorrhage. J Magn Reson Imaging 2009 Jan; 29(1): 31-38. SCI=2.566 (RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING, 31.67%)

  16. Liu CL, Shen CR (equal to first), Hsu FF, Chen JK, Wu PT, Guo SH, Lee WC, Yu FW, Mackey ZB, Turk J, Gross ML. Isolation and identification of two novel SDS-resistant secreted chitinases from Aeromonas schubertiiBiotechnol Prog 2009 Jan-Feb; 25(1): 124-131. SCI=1.853 (FOOD SCIENCE & TECHNOLOGY, 34.68%)

  17. Kang CJ, Chen YJ, Liao CT, Wang HM, Chang JT, Lin CY, Lee LY, Wang TH, Yen TC, Shen CR, Chen IH, Chiu CC, Cheng AJ. Transcriptome profiling and network pathway analysis of genes associated with invasive phenotype in oral cancer. Cancer Lett 2009 284(2):131-140. Epub 2009 May 18. SCI=4.258 (ONCOLOGY, 25.00%)

  18. Yang CJ, Liu YK, Liu CL, Shen CN, Kuo ML, Su CC, Tseng CP, Yen TC, Shen CR (correspondence). Inhibition of acidic mammalian chitinase by RNA interference suppresses OVA-sensitized allergic asthma. Hum Gene Ther 2009 Dec; 20(12): 1597- 1606. Epub 2010 Jun 23. SCI=4.019 (BIOTECHNOLOGY & APPLIED MICROBIOLOGY, 16.35%)

  19. Liu HL, Wai YY, Hsu PH, Lyu LA, Wu JS, Shen CR, Chen JC, Yen TC, Wang JJ. In Vivo assessment of macrophage CNS infiltration during disruption of the blood–brain barrier with focused ultrasound: a magnetic resonance imaging study. J Cereb Blood Flow Metab 2010 Jan; 30(1): 177- 186. Epub 2009 Sep 2. SCI=5.398. (ENDOCRINOLOGY & METABOLISM, 13.22%)

  20. Shen CR, Chen, YS, Yang, CJ, Chen, JK, Liu CL. Colloid chitin azure is a dispersible, low-cost, substrate for chitinase measurements in a sensitive fast reproducible assay. J Biomol Screen 2010 Feb; 15(2): 213- 217. Epub 2009 Dec 30. SCI=2.207 (CHEMISTRY, ANALYTICAL, 42.67%)

  21. Tsai ZT, Wang JF, Kuo HY, Shen CR, Wang JJ, Yen TC. In situ preparation of high relaxivity iron oxide nanoparticle with chitosan coating aspotential MRI contrast agent and cell tracking agent. J Magn Magn Mater 2010, 322: 208–213. SCI=1.826 (MATERIALS SCIENCE, MULTIDISCIPLINARY, 31.80%)

  22. Wu SY, Hsieh CC, Wu RR, Susanto J, Liu TT, Shen CR, Chen Y, Su CC, Chang FP, Chang HM, Tosh D, Shen CN. Differentiation of pancreatic acinar cells to hepatocytes requires an intermediate cell type. Gastroenterology 2010 Jun, 138(7): 2519-2530. Epub 2010 Feb 20. SCI=12.821 (GASTROENTEROLOGY & HEPATOLOGY, 1.35%)

  23. Hsieh CL, Liu SJ, Shen CR, Chen MY, Hsu SC., Tsai JP, Li YS, Yang CJ, Chong P, Chen HW. IL-6-tranfected tumor cells modulate the status of CD8+ and CD4+ T cells to control the tumor growth. Immunobiology 2010 Jun; 215(6): 486- 491. Epub 2009 Aug 19. SCI=2.814 (IMMUNOLOGY, 52.59%)

  24. Shih MF, Cheng YD, Shen CR, Cherng JY. A molecular pharmacology study into the anti-inflammatory actions of Euphorbia hirta L. on the LPS-induced RAW 264.7 cells through a selective iNOS protein inhibition. J Natural Medicine 2010 Jul, 64(3): 330-5. SCI=1.516 (PHARMACOLOGY & PHARMACY, 69.62%) 

  25. Juang JH, Wang, JJ, Shen CR, Kuo CH, Chien YW, Kuo HY, Tsai ZT, Yen TC. Magnetic resonance imaging of transplanted mouse islets labeled with chitosan-coated Superparamagnetic iron oxide nanoparticles. Transplant Proc 2010 Jul-Aug; 42(6): 2104- 2108. SCI=0.952 (SURGERY, 65.66%)

  26. Cherng JY, Liu CC, Shen CR, Chen LY, Shih MF. Beneficial effects of Chlorella-11 peptide on blocking LPS-induced macrophage activation and alleviating thermal injury-induced inflammation in rats. Int J Immunopathol Pharmacol 2010 Jul-Sep; 23(3):811-820. SCI=2.991 (PATHOLOGY, 24.05%)

  27. Liu YK, Yang CJ, Liu CL, Shen CR, Shiau LD. Using a fed-batch culture strategy to enhance rAAV production in the baculovirus/insect cell system. J Biosci Bioeng 2010 Aug; 110(2):187-193. Epub 2010 Mar 11. SCI=1.737 (FOOD SCIENCE & TECHNOLOGY, 38.71%)

  28. Chen JK, Shen CR (equal to first), Liu CL. N-acetyl-glucosamine: Production and Applications (invited review). Mar Drugs 2010 Sep 15; 8(9):2493-2516. SCI=3.978 (CHEMISTRY, MEDICINAL, 10.17%)

  29. Chang JT, Kuo TF, Chen YJ, Chiu CC, Lu YC, Li HF, Wang HM, Shen CR, Cheng AJ. Highly potent and specific siRNAs against E6 or E7 genes of HPV16- or HPV18-infected cervical cancers. Cancer Gene Ther 2010, Dec; 17(12):827-836. Epub 2010 Oct1. SCI=2.945 (BIOTECHNOLOGY & APPLIED MICROBIOLOGY, 30.82%)

  30. Juang JH, Shen CR (equal to first), Wang, JJ, Kuo CH, Lin MY, Wu ST, Tsai ZT, Yen TC. Magnetic resonance imaging study of mouse islet allotransplantation. Transplant Proc 2010 Dec;42(10):4217-20. SCI=0.952 (SURGERY, 65.66%)

  31. Chen JC, Ou LS, Chang ML, Chang PY, Muench MO, Shen CR, Chang HL, Yu HY, Fu RH, Kuo ML. Characterization of tolerance after in utero hematopoietic stem cell transplantation: the requirement for a threshold level of chimerism to establish rather than maintain postnatal skin tolerance. Cell Transplant 2010 Dec;19 (12):1609-22. Epub 2010 Aug 17. SCI=4.422 (TRANSPLANTATION,15.38%)

  32. Hwang YS, Shen CR, Chang SHL, Lai CC, Liu CL, Chen KJ, Lin KK, Chen TL, Hsiao CH. The validity of clinical feature profiles for cytomegaloviral anterior segment infection. Graefes Arch Clin Exp Ophthalmol 2011 Jan; 249:103–110 Epub 2010 Sep 21. SCI=1.932 (OPHTHALMOLOGY, 34.48%)

  33. Chen JK, Shen CR (equal to first), Yeh CH, Fang BS, Huang TL, Liu CL. N-acetyl glucosamine obtained from chitin by chitin degrading factors in Chitinbacter tainanesis. Int J Mol Sci 2011 Feb; 12: 1187-1195. SCI=2.464 (CHEMISTRY, MULTIDISCIPLINARY, 31.58%)

  34. Shen CR (correspondence), Juang JH, Tsai ZT, Wu ST, Tsai FY, Wang JJ, Yen TC. Preparation, characterization and application of superparamagnetic iron oxide encapsulated with N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride. Carbohydr Polym 2011 Mar; 84(1): 781- 787. Epub 2010 Aug. SCI=3.479 (CHEMISTRY, APPLIED, 9.86%)

  35. Lee MH, Chang JT, Yu FW, Shen CR (correspondence). The prevalence of IL-17–producing cells in patients with Nasopharyngeal carcinoma. J Biomed Lab Sci 2011 Mar; 23(1): 30-37.

  36. Hsieh CL, Hsu SC, Shen CR, Chen MY, Liu SJ, Chong P, Chen HW. Increased expression of IL-21 reduces tumor growth by modulating the status of tumor-infiltrated lymphocytes. Immunobiology 2011 Apr; 216: 491–496. Epub 2010 Sep 15. SCI=2.814 (IMMUNOLOGY, 52.59%)

  37. Shen CR, Wu ST, Tsai ZT, Wang JJ, Yen TC, Tsai JS, Liu CL. Characterization of the Quaternized chitosan-stabilized iron-oxide nanoparticles as a novel potential MRI contrast agent for cell tracking. Poly International 2011 Jun 60(6): 945-950. SCI=2.125(POLYMER SCIENCE, 30.12%)

  38. Chen JK, Yeh CH, Wang LC, Liou TH, Shen CR (correspondence), Liu CL. Chitosan, the marine functional food for a potent adsorbent of humic acid. Mar Drugs 2011 9, 2488-2498; doi:10.3390/md9122488. SCI=3.978 (CHEMISTRY, MEDICINAL, 10.17%)

  39. Wang CH, Hwang YS, Chiang PR, Shen CR, Hong, WH, Hsiue GH. Extended release of Bevacizumab by thermosensitive biodegradable and biocompatible hydrogel. Biomacromolecules 2012;13(1):40-8. Epub 2011 Dec 6. dx.doi.org/ 10.1021/ bm 2009558 | SCI=5.371 (POLYMER SCIENCE, 4.82%)

  40. Tsai JP, Lee MH, Hsu SC, Chen MY, Liu SJ, Chang JT, Liao CT, Cheng AJ, Chong P, Shen CR (correspondence), Chen HW. CD4+ T cells disarm or delete cytotoxic T lymphocytes under IL-17-polarizing conditions. J Immunol 2012 189:1671-1679 SCI=5.520 (IMMUNOLOGY, 17.78%)

  41. Wu CJ, Huang WC, Chen LC, Shen CR, Kuo ML. Pseudotypedadeno-associated virus2/9-deliveredCCL11 shRNA alleviateslung inflammationinan allergen-sensitized mouse model. Hum Gene Ther 2012, 23(11): 1156-1165. doi:10.1089/hum.2012.012. SCI=4.019 ((BIOTECHNOLOGY & APPLIED MICROBIOLOGY, 16.35%)

  42. Tsai ZT, Tsai FJ, Yang WC, Wang JF, Liu CL, Shen CR (correspondence), Yen TC. Preparation and characterization of ferrofluid stabilized with biocompatible chitosan and dextran sulfate hybrid biopolymer as a potential MRI T2 contrast agent. Mar Drugs 2012; 10(11), 2403-2414. SCI=3.978 (CHEMISTRY, MEDICINAL, 10.17%)

  43. Fan CH, Chen CY, Chen KT, Shen CR, Kuo YB, Chen YS, Chou YP, Wei WS, Chan EC. Blockade of phospholipid scramblase 1 with its N-terminal domain antibody reduces tumorigenesis of colorectal carcinomas in vitro and in vivo. 2012. J Transl Med. 2012 Dec 24;10:254. doi: 10.1186/1479-5876-10-254. SCI=3.459 (MEDICINE, RESEARCH & EXPERIMENTAL, 29.75%)

  44. Hsu PH, Wei KC, Huang CY, Wen CJ, Yen TC, Liu CL, Lin YT, Chen JC, Shen CR (correspondence), Liu HL. Noninvasive and targeted gene delivery into the brain using microbubble-facilitated focused ultrasound. PLoS One 2013 Feb; 8(2): e57682. SCI=3.730 (MULTIDISCIPLINARY SCIENCES, 12.50%)

  45. Shen CR, Liu CL, Lee HP, Chen JK. The identification and characterization of chitotriosidase activity in pancreatin from porcine pancreas. Molecules 2013 Mar; 148, 2978-2987. SCI=2.428 (CHEMISTRY, ORGANIC, 41.82%)

  46. Juang JH, Shen CR (equal to first), Wang, JJ, Kuo CH, Chien YW, Kuo HY, Chen FR, Chen MH, Yen TC, Tsai ZT. Magnetic resonance imaging of mouse islet grafts labeled with novel chitosan-coated superparamagnetic Iron Oxide nanoparticles. PLoS One 2013 April; 8(4):e62626. SCI=3.730 (MULTIDISCIPLINARY SCIENCES, 12.50%)

  47. Shen CR, Chen HW. Fatal attraction: tumor recruitment of myeloid-derived suppressor cells is mediated by IL-17-producing CD8+ T cells. Transl Gastrointest Cancer 2013 June;2(S1):119-122. doi: 10.3978/j.issn.2224-4778.2013.05.25

  48. Hwang YS, Chiang PR, Hong WH, Chiao CC, Chu IM, Hsiue GH, Shen CR (correspondence). Study in vivo intraocular biocompatibility of in situ gelation hydrogels: Poly (2-ethyl oxazoline)- block- poly (<epsilon>- caprolactone)- block- poly (2-ethyloxazoline) Copolymer, Matrigel and Pluronic F127. PLoS One 2013 July; (7): e67495. SCI=3.730 (MULTIDISCIPLINARY SCIENCES, 12.50%)

  49. Chen YS, Liu CL, Lee MH, Chen HW, Shen CR (correspondence). The role of Tc17 in cancer immunity and clinical associations. J Biomed Lab Sci 2013 Sep; 25(3): 77-83.

  50. Huang HI, Chen SK, Wang RYL, Shen CR, Cheng YC. Human foreskin fibroblast-like stromal cells can differentiate into functional hepatocytic cells. Cell Biol Int 2013 16 Sep; 37: 1308-1319. SCI=1.640 (BIOPHYSICS, 81.52%)

  51. Lin YH, Chang HM, Chang FP, Shen CR (equal to second), Liu CL, Mao WY, Lin CC, Shen CN. Protoporphyrin IX accumulation disrupts mitochondrial dynamics and function in ABCG2-deficient hepatocytes. FEBS Letters 2013 1 Oct; 587(19): 3202-3209. SCI=3.582 (BIOPHYSICS, 25.00%)

  52. Shen CR, Yang WC, Chen HW. The fate of regulatory T cells: survival or apoptosis. Cellular & Molecular Immunology 2014 Jan; 11(1):11-13. doi:10.1038/cmi.2013.49. SCI=3.419 (IMMUNOLOGY, 37.78%)

  53. Chen JK, Shen CR (equal to first), Liu CL. The characteristics of chitinase expression in Aeromonas schubertii. Appl Biochem Biotechnol 2014 Apr; 172(8): 3827-3834.  doi:10.1007/s12010-014-0798-1. SCI=1.893 (BIOTECHNOLOGY & APPLIED MICROBIOLOGY, 54.37%)

  54. Chen YS, Shen CR (correspondence). Immune checkpoint blockade therapy: The 2014 Tang Prize in Biopharmaceutical Science. Biomed J 2015 38:5-8. doi: 10.4103/2319-4170.151150. 

Book and Book Chapter:

  1. Elson, CJ, Shen, CR (1998) Anemia, autoimmune hemolytic in experimental model. In Encyclopedia of Immunology (p.91-93), 2nd Ed. Edited by Roitt, I.M. and Delves, P.J. Arnold.

  2. Shen, CR (1998) T-cell specificity in autoimmune haemolytic anaemia. Ph.D. thesis, Univeristy of Bristol, University of Bristol, UK.

  3. 臨床血清免疫學PBL教案/張若南主編;沈家瑞等編著(2003)/國立陽明大學

  4. 臨床血清免疫學/Mary Louis Turgeon 原著; 郭永斌等編譯; 沈家瑞等審閱(2004)/合記出版社

  5. Advances in Chitin Science- vol XII, p196-199. (2010) Published by FEQ-UNICAMP , Brazil (ISBN978-85-64131-00-2)

  6. 藥理學原理:以病態生理學為基礎的藥物治療(Principles of Pharmacology:The Pathophysiologic Basis of Drug Therapy, 3/e)/ Golan D.E et al. 原著;沈家瑞等編譯(2015)/藝軒出版社 (ISBN 978-95-76169-98-4)

Patents:

  1. 劉昭麟、林楷洺、劉裕國、陳錦坤、沈家瑞(2009)單離之幾丁質水解蛋白質、DNA、重組載體、重組幾丁質水解蛋白質以及表現系統。中華民國發明專利第I314557號。

  2. 劉昭麟、陳昱昇、陳錦坤、沈家瑞(2010)幾丁質酵素的快速且多樣化定性試劑組與方法。中華民國發明專利第I 322889號。

  3. Liu CL, Yang CJ, Shen CR (2010) Development of Asthma Therapy with rAAV-mediated AMCase shRNA. USA Patent US7,851,616.

  4. 劉昭麟、楊靜仁、沈家瑞(2012)短片段干擾RNA(siRNA)核苷酸及其醫藥組合物及用途。中華民國發明專利第I378800號。

  1. 劉昭麟、沈家瑞、陳力振(2015)利用幾丁質引發之免疫反應檢測過敏性氣喘病人之檢驗方法。中華民國發明專利第I479152號。

Liu CL, Chen LC, Shen CR. (2015) A Chitin-induced Immune Response Based Method for Diagnosing Allergic Asthma in Patients. USA Patent US9,005,899.

 

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