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顏秀娟 教授

顏 秀 娟 (Hsiu-Chuan Yen)

學歷

美國肯塔基大學毒理學博士

 


 

任課科目

大學部(臨床毒物學、臨床生化學、自由基生物學概論、細胞生物學)、碩士班(自由基生物醫學、氧化還原生物學特論、高等生物技術學)

研究專長

自由基生物醫學、粒線體生物醫學、毒理學、臨床生化學

辦公室分機

5207

實驗室分機

5207

個人網頁

自由基暨粒線體醫學研究室

E-mail

yen@mail.cgu.edu.tw

研究方向及研究室特色(Lab & Research Interest)

1. 在台灣建立唯一用氣相層析/負離子化學游離質譜分析之參考方法來偵測體液、組織或細胞中isoprostanes、neuroprostanes及其相關分子之平台。這些分子為活體中最專一之脂質過氧化指標,而這樣之平台則主要用於研究各式人類疾病和氧化傷害之關係。

2. 藉由偵測病人腦脊髓液和血漿中之F2-isoprostanes、F4-neuroprostanes、nitrate/nitrite和多種脂溶性抗氧化物質之含量,我們探討氧化壓力和蜘蛛膜下腔出血(一種常見之出血性中風)病人或頭部外傷病人之氧化壓力和癒後/臨床嚴重性的關係。

3. 實驗性蜘蛛膜下腔出血所引發之血管痙攣在大鼠中造成之氧化壓力及超氧歧化酶/輔酶模擬物之可能保護作用。

4. 以定序、PCR-RFLP、denaturing HPLC和pyrosequencing偵測不同惡性人類星狀細胞瘤組織及其復發組織之體細胞粒線體DNA突變。

5. 使用人類細胞株或cybrids,探討內生性coenzyme Q10含量和合成coenzyme Q10所需之PDSS或COQ基因對氧化壓力或粒線體功能缺失所產生之反應及調控。

6. 內生性coenzyme Q10 末端合成路徑所需人類COQ蛋白(COQ2-COQ9)之研究。

論文與著作(Publication)

近五年所發表論文(其他更多論文請見實驗室網頁)

  1. H-C Yen*, S-H Li, H J Majima, Y-H Huang, C-P Chen, Y-C Tu, and C-W Chen. 2011 June. Up-regulation of antioxidant enzymes and coenzyme Q10 in a human oral cancer cell line with acquired bleomycin resistance. Free Radical Research, 45: 707-716. [SCI]
  2. H J Majima*, H P Indo, S Suenaga, H Matsui, H-C Yen, T Ozawa. 2011 July. Mitochondria as possible pharmaceutical targets for the effects of vitamin E and its homologues in oxidative stress-related diseases. Current Pharmaceutical Design, 17: 2190-2195. [SCI]
  3. H-C Yen*, F-Y Chen, S-W Chen, Y-H Huang, Y-R Chen, C-W Chen. 2011 September. Effect of mitochondrial dysfunction and oxidative stress on endogenous levels of coenzyme Q10 in human cells. Journal of Biochemical and Molecular Toxicology, 25: 280-289. [SCI]
  4. Y-K Lin, H-W Chen, S-H Yang, Y-L Leu, Y-H Huang, H-C Yen*. 2012 February. Protective effect of indigo naturalis extract against oxidative stress in cultured human keratinocytes. Journal of Ethnopharmacology, 139: 893-896. [SCI]
  5. H P Indo, O Inanami, T Koumura, S Suenaga, H-C Yen, S. Kakinuma, K C Matsumoto, I Nakanishi, W St. Clair, D K St. Clair, H Matsui, R Cornette, O Guesev, T Okuda, Y Nakagawa, T Ozawa, H J Majima*. 2012 August. Roles of mitochondria-generated reactive oxygen species on X-ray-induced apoptosis in a human hepatocellular carcinoma cell line, HLE. Free Radical Research, 46: 1029-1043. [SCI]
  6. H P Indo, I Nakanishi, K Ohkubo, H-C Yen, M Nyui, S Manda, K-I Matsumoto, K Fukuhara, K Anzai, N Ikota, H Matsui, Y Minamiyama, A Nakajima, H Ichikawa, S Hukuzumi, T Ozawa, C Mukai, H J Majima*. 2013 March. Comparison of in vivo and in vitro antioxidative parameters for eleven food factors. RSC Advances, 3: 4535-4538. [SCI)
  7. S-W Chen, C-C Liu, H-C Yen*. 2013 March. Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5. Mitochondrion, 13: 143-152. [SCI]
  8. 顏秀娟*. 2013 June. 內生性輔酶Q之淺談. 生物醫學, 6:103-110.
  9. H-C Yen*, H-J Wei, and T-W Chen. 2013 July. Analytical variables affecting analysis of F2-isoprostanes and F4-neuroprostanes in human cerebrospinal fluids by gas chromatography/mass spectrometry. Biomedical Research International, 2013: Article 810915 (1-14). [SCI]
  10. H-C Yen*, S-L Li, W-C Hsu, P Tang. 2014 March. Interference of co-amplified nuclear mitochondrial DNA sequences on the determination of human mtDNA heteroplasmy by using the SURVEYOR Nuclease and the WAVE HS system. PLOS ONE, 9: e92817 (1-18). [SCI]
  11. H-C Yen*, C-C Liu, C-C, Kan, C-S Chen, H-R Wei. 2014 September. Suppression of coenzyme Q10 levels and the induction of multiple PDSS and COQ genes in human cells following oligomycin treatment. Free Radical Research, 48: 1125-1134. [SCI]
  12. H P Indo, H-C Yen, I Nakanishi, K-I Matsumoto, M Tamura, Y Nagano, H Matsui, O Gusev, R Cornette, T Okuda, Y Minamiyama, H Ichikawa, S Suenaga, M Oki, T Sato, T Ozawa, D K St.Clair, H J Majima*. 2015 January. A mitochondrial superoxide theory for oxidative stress diseases and aging. Journal of Clinical Biochemistry and Nutrition, 56: 1-7. [SCI]
  13. H-C Yen*, H-J Wei, C-L Lin. 2015 July. Unresolved issues in the analysis of F2-isoprostanes, F4-neuroprostanes, isofurans, neurofurans, and F2-dihomo-isoprostanes in body fluids and tissue using gas chromatography/negative-ion chemical-ionization mass spectrometry. Free Radical Research, 49: 861-880. [SCI]
  14. H-C Kuo*, H-C Yen, C-C Huang, W-C Hsu, H-J Wei, C-L Lin. 2015 October. Cerebrospinal fluid biomarkers for neuropsychological symptoms in early stage of late-onset Alzheimer's disease. International Journal of Neuroscience, 125: 747-754. [SCI]
  15. H-C Yen, T-W Chen, T-C Yang, H-J Wei, J-C Hsu*, C-L Lin*. 2015 December. Levels of F2-isoprostanes, F4-neuroprostanes, and total nitrate/nitrite in plasma and cerebrospinal fluid of patients with traumatic brain injury. Free Radical Research, 49: 1419-1430. [SCI]
  16. H-C Yen*, Y-C Liu, C-C Kan, H-J Wei, S-H Lee, Y-H Wei, Y-H Feng, C-W Chen, C-C Huang. Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency. Biochimica et Biophysica Acta, accepted on May 2016. [SCI]

(* 負責/通訊作者)

 

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