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施信如教授
 

施 信 如 (Shin-Ru Shih)

學歷

美國紐澤西羅格斯大學分子生物及生物化學博士

任課科目

臨床病毒學(實驗及實習) 、分子生物學、細胞生物學

研究專長

Clinical Virology、Biotechnology、Molecular Biology、Biochemistry

辦公室分機

5497

實驗室分機

5497

個人網頁

分子病毒實驗室

E-mail

srshihmail.cgu.edu.tw

研究方向及研究室特色(Lab & Research Interest)

 

我們實驗室主要致力於新興病毒的研究,尤其是流感病毒及腸病毒71型這兩個容易有變異的RNA病毒。研究的方向包括(1)新型(突變株)病毒的檢驗(2)病毒與宿主互動的研究(3)抗病毒藥物的研發。

在新型病毒的檢驗方面,我們以特別的細胞株(例如某種基因缺陷或大量表達的細胞株)來培養病毒,加上各種不同的分子檢驗方式偵測鑑定新型病毒。而在病毒與宿主互動的研究方面,我們致力於宿主蛋白質如何調控病毒基因的複製及病毒蛋白質如何干擾宿主細胞基因的表達與調控,藉由病毒與宿主互動的研究,讓我們了解流感病毒跨越宿主的機制,也讓我們了解腸病毒71型的分子致病機轉。而病毒與宿主互動的研究,也讓我們從中發掘重要的分子標的,進一步研發抗病毒藥物。

 

論文與著作(Publication)

 五年內論文:

  1. Chen LL, Kung YA, Weng KF, Lin JY Horng JT and *Shih SR. Enterovirus 71 infection cleaves negative regulator for viral internal ribosomal entry site-driven translation. Journal of Virology, 2013, 87(7);3828-3838.

  2.  Huang CH, Chen CJ, Yen CT, Yu CP, Huang PN, Kuo RL, Lin SJ, Chang CK and *Shih SR. Caspase-1 deficient mice is more susceptible to influenza A virus infection with PA variation. Journal of Infectious Diseases. 2013: 208(11):1998-1905.

  3. Mok CK, Chen GW, Shih KC, Gong YN, Lin SJ, Horng JT, Hsu TA, Chen CJ and *Shih SR. Evolutionarily Conserved Residues at an Oligomerization Interface of the Influenza A Virus Neuraminidase are Essential for Viral Survival. Virology, 2013; 447(1-2):32-44. doi: 10.1016. (highly down loaded in Elsevier, 557 times in the first year after publication)

  4. Liu YC, Kuo RL, Lin JY, Huang PN, Huang Y, Liu H, Arnold, JJ, Chen SJ, Wang, RY, Cameron CE and *Shih SR. Cytoplasmic viral RNA-dependent RNA polymerase disrupts the intracellular splicing machinery by entering nucleus and interfering with Prp8. PLoS Pathogens, 2014; 10(6):e1004199.

  5. Huang PN and *Shih SR. Update on enterovirus 71 infection. Current Opinion in Virology, 2014; 98-104. doi: 10.1016/j.coviro.2014.03.007..

  6. Kung YA, CT Hung, YC Liu and *Shih SR. Update on the development of enterovirus 71 vaccines. Expert Opinion On Biological Therapy, 2014; 14(10):1455-64.

  7. Weng KF, Hung CT, Hsieh PT, Li ML, Chen GW, Kung YA, Huang PN, Kuo RL, Chen LL, Lin JY, Wang RYL, Chen SJ, Tang P, Horng JT, Huang HI, Wang JR, Ojcius DM, Brewer G and *Shih SR. A Cytoplasmic RNA Virus Generates Functional Viral Small RNAs and Regulates Viral IRES Activity in Mammalian Cells. Nucleic Acids Research, 2014 Nov 10;42(20):12789-805.

  8. Balaji VA, Chen GW, Hsieh PT, Horng JT, Hsu JT, Hsieh HP, Chen TC, Weng KF and *Shih SR. BPR-3P0128 inhibits RNA-dependent RNA polymerase elongation and VPg uridylylation activities of enterovirus 71. Antiviral Research, 2014 Dec;112:18-25.

  9. Chen YH, Chang GK, Kuo SM, Huang SY, Hu IC, Lo YL,*Shih SR. Well-tolerated Spirulina extract inhibits influenza virus replication and reduces virus-induced mortality. Sci Rep. 2016 Apr 12;6:24253. doi: 10.1038/srep24253.

  10. Chen MF, Weng KF, Huang SY, Liu YC , Tseng SN ,David M. Ojcius, *Shih SR . Pretreatment with a heat-killed probiotic modulates monocyte chemoattractant protein-1 and reduces the pathogenicity of influenza and enterovirus 71 infections. Mucosal Immunology. 016 Apr 13. doi: 10.1038/mi.2016.31. [Epub ahead of print]

  11.  Hung CT, Kung YA, Lai ML, Brewer G, Liu ST, *Shih SR. Additive promotion of viral internal ribosomal entry site-mediated translation by far upstream element-binding protein 1 and an enterovirus 71-induced cleavage product. PLoS Pathogens. 2016 Oct 25;12(10):e1005959. doi: 10.1371/ journal.ppat.1005959.

  12. Kung YA, Hung CT, Chien KY, *Shih SR Control of the downregulating IRES trans-acting factor KSRP by ubiquitination. Nucleic Acids Res., 2017, 45:1, 271-287. (doi: 10.1093/nar/gkw1042.)

  13. Lee KM, Chen CG, *Shih SR. Viral internal ribosome entry site-dependent translation. Trends in Microbiology, 2017, 25:7, 546-561. (pii: S0966-842X(17)30022-7. doi: 10.1016/j.tim.2017.01.010.) 

  14.  Huang PN, Jheng JR, Arnold JJ, Wang JR, Cameron CE, *Shih SR. UGGT1 enhances enterovirus 71 pathogenicity by promoting viral RNA synthesis and viral replication. PLoS Pathogens. 2017 May 17;13(5):e1006375. doi: 10.1371/journal.ppat.1006375. eCollection 2017 May.PMID:28545059

  15. Kuo SM, Chen CJ, Chang SC, Liu TJ, Chen YS, Huang SY, *Shih SR. Inhibition of avian influenza A virus replication in human cells by host restriction factor TUFM is correlated with autophagy. mBio, in press, 2017. 

 

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