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研究方向及研究室特色(Lab & Research Interest)

Molecular analysis of the interplay between tumor microenvironment and cancer progression

      Cancer is one of the major causes of death worldwide and has a significant impact on national health. During cancer progression, tumor cells are confronted with platelet and various plasma proteins in the bloodstream that has been defined as the third microenvironment in addition to the primary tumor site (the primary tumor microenvironment) and the distant metastatic organs (the second tumor microenvironment). Experimental and clinical data all indicate that tumor cell interacting with platelets and the clotting system are important elements of tumor progression and cancer cells metastasis leading to the development of advanced stage cancer. Abrogation of cancer cell-platelet interaction thereby provides an attractive target for development of innovative cancer therapeutic regimens. We aim to identify novel regulators that are crucial for cancer cell-platelet interactions using various molecular and cellular approaches. A number of in vivoand in vitro study models have been established that can be applied for target gene discovery and analysis. Several regulators that mediate cancer cell-platelet interactions are candidates for translating to clinical application and are under intensive investigation.

Molecular analysis of platelet function and platelet biogenesis

      Platelet is important in various physiological and pathological conditions, including hemostasis, thrombosis, and cancer. Understanding the mechanistic insight of platelet biogenesis and signaling can contribute to the development of effective therapy for thrombosis, thrombocytopenia, and cancer. Although it has been known for years that each megakarycoyte produces about 5000 platelets through the formation of proplatelet with thin tread structure protruding from the megakaryocytes, the key regulators in the control of megakaryocytic differentiation and platelet biogenesis are not yet completely understood. At present, the murine embryonic stem cells-OP9 coculture system and the lineage-specific DAB2 knockout mice are used as the study model to elucidate the functional role of the adaptor protein Disabled-2 (DAB2) in the regulation of megakaryocytic differentiation, proplatelet formation and platelet function. We expect to gain new insight on the mechanisms underlying megakaryocytic maturation and platelet biogenesis that should provide information on how functional platelets are produced in physiological condition and how to obtain and maintain appropriate platelet count underpathological condition.




  1. Tseng C-P, Ely BD, Li Y, Pong RC, and Hsieh JT. Regulation of rat DOC-2 gene during castration-induced rat ventral prostate degeneration and its growth inhibitory function in human prostatic carcinoma cells. Endocrinology 139:3542-3553, 1998. (SCI)
  2. Li Y, Pong RC, Hall MC, Tseng C-P, Wang Z, Sagalowsky AL, Bergelson JM, and Hsieh JT. Lack of adenoviral receptor expression in human bladder cancer cells – a potential impact on the efficacy of gene therapy. Cancer Res. 59:325-330, 1999. (SCI, IF = 7.514)
  3. Tseng C-P, Ely BD, Pong RC, Li Y, Wang Z, Cobb MH, and Hsieh JT. The role of rat DOC-2 protein phosphorylation in the negative regulation of AP-1 activity – an underlying mechanism of its tumor suppressive function. J. Biol. Chem. 274:31981-31986, 1999. (SCI, IF = 5.520)
  4. Reddig PJ, Dreckschimdt NE, Ahrens H, Simsiman R, Tseng C-P, Zou J, Oberley T, and Verma AK. Transgenic mice overexpressing protein kinase C in the epidermis are resistant to skin tumor promotion by 12-O-tetradecanoylphorbol-13-acetate (TPA). Cancer Res. 59:5710-5718, 1999. (SCI, IF = 7.514)
  5. Lin M-H, Chen T-C, Kuo T-T, Tseng C-C, and Tseng C-P (Corresponding author). Development of real-time quantitative polymerase chain reaction for the detection of Toxoplasma gondii. J. Clin. Microbiology 38:4121-4125, 2000. (SCI)
  6. Tseng C-P (Corresponding author), Huang C-H, Tseng C-C, Lin M-H, Hsieh JT, and Tseng C-H. Induction of Disabled-2 gene during megakaryocyte differentiation of K562 cells. Biochem. Biophys. Res. Commun. 285:129-135, 2001. (SCI)
  7. Lin M-H, Tseng C-H, Tseng C-C, Huang C-H, Chong C-K, and Tseng C-P(Corresponding author). Real-time PCR for rapid genotyping of angiotensin-converting enzyme insertion/deletion polymorphism. Clin. Biochem. 34:661-666, 2001. (SCI)
  8. Chu DC, Chuang CK, Fu JB, Huang H-S,Tseng C-P, and Sun CF. The use of real-time quantitative polymerase chain reaction to detect hypermethylation of the CpG islands in the promoter region flanking the GSTP1 gene to diagnose prostate carcinoma. J. Urology. 167:1854-1858, 2002. (SCI)
  9. Wang Z, Tseng C-P, Pong RC, Chen H, McConnell JD, Navone N, and Hsieh JT. The mechanism of growth inhibitory effect of DOC-2/DAB2 in prostate cancer: Characterization of a novel GTPase activating protein associated with N-terminal domain of DOC-2/DAB2. J. Biol. Chem. 277:12622-12631, 2002. (SCI, IF = 5.520)
  10. Tseng C-P (Corresponding author), Cheng AJ, Chang JTC, Tseng CH, Wang HM, Liao CTL, Chen IH, and Tseng CC. Development of real-time RT-PCR for quantitative analysis of multidrug resistant mdr1 gene expression in oral cancer. Japanese J. Cancer Res. 93:1230-1236, 2002. (SCI)
  11. Tseng C-P, Cheng JC, Tseng CC, Wang C, Chen YL, Chiu DTY, Liao HC, and Chang SS. Broad-range ribosomal RNA real-time PCR after removal of DNA from reagents: melting profiles for clinically important bacteria. Clin. Chem. 49:306-309, 2003. (SCI, IF = 5.579)
  12. Tseng C-P (corresponding author), Huang CL, Huang CH, Stern A, Cheng JC, Tseng CH, and Chiu DTY. Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells. FEBS Letters 541:21-27, 2003. (SCI)
  13. Chan YL*,Tseng C-P*, Tsay PK, Chang SS*, Chiu TF, and Chen JC. Procalcitonin as a marker of bacterial infection in the emergency department: an observational study. Critical Care 8:R12-R20, 2004. (*Co-first authors)
  14. Huang CL, Cheng JC, Liao CH, Stern A, Hsieh JT, Wang CH, Hsu HL, andTseng C-P (Corresponding author). Disabled-2 is a negative regulator of integrin IIb3-mediated fibrinogen adhesion and cell signaling. J. Biol. Chem. 279:42279-42289, 2004. (SCI, IF = 5.520)
  15. Zhou J, Hernandez G, Tu SW, Scholes J, Chen H, Tseng C-P, Hsieh JT. Synergistic induction of DOC-2/DAB2 gene expression in transitional cell carcinoma in the presence of GATA6 and histone deacetylase inhibitor. Cancer Res. 65:6089-6096, 2005. (SCI, IF = 7.514)
  16. Tseng C-P (Corresponding author), Chang P, Huang CL, Cheng JC, and Chang SS. Autocrine signaling of platelet-derived growth factor regulates Disabled-2 expression during megakaryocytic differentiation of K562 cells. FEBS Letters, 579:4395-4401, 2005. (SCI)
  17. Tseng C-P, Huang CL, Chong KY, Hung IJ, and Chiu DTY. Rapid detection of glucose-6-phosphate dehydrogenase gene mutations by denaturing high-performance liquid chromatography. Clin. Biochem. 38:973-980, 2005. (SCI)
  18. Zhou J, Hernandez G, Tu SW, Huang CL, Tseng C-P, and Hsieh JT. The role of DOC-2/DAB2 in modulating androgen receptor-mediated cell growth via the nongenomic c-Src-mediated pathway in normal prostatic epithelium and cancer. Cancer Res. 65:9906-9913, 2005. (SCI, IF = 7.514)
  19. Huang CL, Cheng JC, Stern A, Hsieh JT, Liao CH, Tseng C-P (Corresponding author). Disabled-2 is a novel integrin IIb-binding protein that negatively regulates platelet-fibrinogen interactions and platelet aggregation. J. Cell Sci. 119:4420-4430, 2006. (SCI, IF = 6.247)
  20. Cheng JC, Huang CL, Lin CC, Chen CC, Chang YC, Chang SS, Tseng C-P(Corresponding author). Rapid detection and identification of clinically important bacteria by high-resolution melting analysis after broad-range ribosomal RNA real-time PCR. Clin. Chem. 52:1997-2004, 2006. (SCI, IF = 5.579)
  21. Huang CH, Cheng JC, Chen JC, Tseng C-P (Corresponding author). Evaluation of the role of Disabled-2 in nerve growth factor-mediated neurite outgrowth and cellular signaling. Cell. Signal., 19:1339-1347, 2007. (SCI)
  22. Lin JH*, Tseng C-P*, Chen YJ, Lin CY, Chang SS, Wu HS, Cheng JC. Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis. J. Clin. Microbiol. 46:1090-1097, 2008 (SCI, *Co-first authors)
  23. Wang SY*, Tseng C-P*, Tsai KC, Lin CF, Wen CY, Tsay HS, Sakamoto N, Tseng CH, Cheng JC. Bioactivity-guided screening identifies pheophytin a as a potent anti-hepatitis C virus compound from Lonicera hypoglauca Miq. Biochem. Biophys. Res. Commun. 385:230-235, 2009. (Co-first authors)
  24. Chen DP*, Tseng C-P*, Tsai SH, Wang MC, Lu SC, Wu TL, Chang PY, Sun CF. Use of X-linked short tandem repeats loci to confirm mutations in parentage caseworks. Clin. Chim. Acta. 408:29-33, 2009. (*Co-first authors)
  25. Yang CM, Hsieh HL, Yao CC, Hsiao LD, Tseng C-P, Wu CB. Protein Kinase C- transactivates platelet-derived growth factor receptor- in mechanical strain-induced collagenase 3 (matrix metalloproteinase-13) expression by osteoblast-like cells. J. Biol. Chem. 284:26040-26050, 2009. (SCI, IF = 5.520)
  26. Tseng WL, Huang CL, Cheng JC, Liao CH, Stern A, Tseng C-P(Corresponding author). Reelin is a platelet protein and functions as a positive regulator of platelet spreading on fibrinogen. Cell. Mol. Life Sci. 2010 (In press) (SCI, IF = 5,511).
  27. Huang CL, Cheng JC, Kitajima K, Nakano T, Chong KY, Tseng C-P(Corresponding author). Disabled-2 is required for mesoderm differentiation of murine embryonic stem cells. J. Cell. Physiol. 2010. (SCI) (In revision)


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