自由基暨粒線體醫學實驗室_研究方向 Research Direction

更新日期 : 2024.07.22

基礎研究 (Basic Research)

Regulation of endogenous coenzyme Q₁₀(CoQ₁₀) levels and human PDSS or COQ genes and proteins essential for biosynthesis of CoQ₁₀ in response to oxidative stress or mitochondrial dysfunction in human cell lines or cybrids.
Regulation of endogenous coenzyme Q₁₀(CoQ₁₀) levels and human PDSS or COQ genes essential for the biosynthesis of coenzyme Q₁₀ in response to oxidative stress or mitochondrial dysfunction in human cell lines or cybrids harboring pathogenic mutations of mitochondrial DNA (mtDNA).
Characterization of human PDSS and COQ proteins and mouse Pdss and Coq proteins and identification of high-molecular-weight protein complexes in the mitochondria.

臨床及轉譯研究 (Clinical and Translational Research)

Establishment of the only platform available in Taiwan to detect F₂-isoprostanes, isofurans, and F₄-neuroprostanes, which are the most specific markers of lipid peroxidation in vivo, in body fluids (CSF, plasma, urine), and tissues by the gas chromatography/negative-ion-chemical-ionization mass spectrometry (GC/NICI-MS) method for studying the relationship between various human conditions and oxidative damage. The research topics that have been applied this platform in collaboration with clinicians at the Linkou Chang Gung Memorial Hospital are aneurysmal subarachnoid hemorrhage (a common type of hemorrhagic stroke), traumatic brain injury, Alzheimer’s disease, paraquat (an herbicide) poisoning, and lead (Pb) poisoning.
The investigation on the correlation between the changes related to primary antioxidant enzymes, CoQ₁₀ , and somatic mtDNA mutations and grades or malignancy of human astrocytoma tissues.

近年來執行計畫 (Research Projects in Recent Years)

補助機構 (Funding sources)

CGMH : Chang Gung Memorial Hospital; 長庚醫院

NSC/MOST :National Science Council/Ministry of Science and Technology; 國科會/科技部

補助機構 (Funding sorces)	計畫名稱 (Name of Projects)	起迄年月 (Periods)	擔任主持人(PI) / 共同主持人 (Co-PI)
MOST	外加輔酶Q對PDSS或COQ基因默化在人類細胞中所造成之內生性輔酶Q含量下降、COQ蛋白或COQ蛋白複合體不穩定、及粒線體功能異常的恢復作用	2017.08~ 2020.07	PI
	Restoration of endogenous coenzyme Q levels, stability of COQ proteins or COQ protein complex, and mitochondrial functions in human cells suppressed by the knockdown of PDSS or COQ genes after supplementation of exogenous coenzyme Q
CGMH	比較有無巴拉刈中毒小鼠之肺臟、心臟、及肝臟組織中脂質過氧化產物、脂溶性抗氧化物、及抗氧化酵素之含量	2018.02~ 2019.07	PI
	Comparison on the levels of lipid peroxidation products, lipid-soluble antioxidants, and antioxidant enzymes in lung, heart, and liver tissues from mice without versus with paraquat poisoning
CGMH	免疫抑制治療對巴拉刈中毒小鼠各組織中脂質過氧化程度及內生性coenzyme Q含量之影響	2019.08~ 2022.01	PI
	Effect of immunosuppressive therapy on the extent of lipid peroxidation and endogenous coenzyme Q levels in various tissues of paraquat-poisoned mice
MOST	人類細胞中各個PDSS和COQ蛋白之特性分析及其與粒線體功能失常或氧化壓力的關聯性	2020.08~ 2023.07	PI
	Characterization of various PDSS and COQ proteins in human cells and its relationship with mitochondrial dysfunction or oxidative stress
CGMH	慢性鉛中毒後小鼠腎臟和血漿中脂質過氧化和 coenzyme Q狀態之變化及 Dimaval 螯合治療之可能保護作用	2022.10~ 2024.09	PI
	Changes in the status of lipid peroxidation and coenzyme Q in mouse kidneys and plasma following chronic lead poisoning and the potential protective effect of the Dimaval chelation therapy
NSC	小鼠細胞和組織之Pdss2和多個Coq蛋白的特性分析與含這些蛋白之蛋白複合體的鑑定	2023.08~ 2024.07	PI
	Characterization of Pdss2 and multiple Coq proteins and identification of protein complexes containing these proteins in mouse cells and tissues
NSC	小鼠細胞和組織之粒線體中coenzyme Q合成體的鑑定及特性分析	2024.08~ 2025.07	PI
	Identification and characterization of coenzyme Q synthome in the mitochondria of mouse cells and tissues
CGMH	巴拉刈中毒後特異性脂質過氧化指標F₂-isoprostanes和脂溶性抗氧化物之偵測	2017.07~ 2019.06	Co-PI （主持人為林口長庚腎臟科顏宗海醫師）
	Detection of specific lipid peroxidation marker F₂-isoprostanes and lipid-soluble antioxidants in paraquat intoxication
CGMH	評估慢性鉛腎變之氧化壓力與發炎反應程度	2019.08~ 2022.07	Co-PI （主持人為林口長庚腎臟科顏宗海醫師）
	Level of oxidative stress and inflammation in chronic lead nephropathy

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