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自由基暨粒線體醫學實驗室_研究方向 Research Direction

 
 
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更新日期 : 2022.07.04
 

基礎研究 (Basic Research)

  1. Regulation of endogenous coenzyme Q10 levels, mRNA levels of PDSS and COQ genes, and protein levels of PDSS and COQ proteins in response for the terminal biosynthesis of Q10 in response to oxidative stress or mitochondrial dysfunction in human cells.
  2. Alteration in the coenzyme  Q10 levels and related events in cybrids with pathogenic mitochondrial DNA mutations from patients.
  3. Effect of exogenous coenzyme Q on the restorations of endogenous coenzyme Q10 and PDSS or COQ proteins in human cells with coenzyme Q10 deficiency.

 

 

臨床及轉譯研究 (Clinical and Translational Research)

  1. Establishment of the only reference method available in Taiwan to detect F2-isoprostanes, F4-neuroprostanes, and isofurans, which are the most specific markers of lipid peroxidation in vivo, in biological fluids, tissues, or cells by gas chromatography/negative-ion-chemical-ionization mass spectrometry (GC/NICI-MS) in order to study diseases and oxidative damage.
  2. The study of the relationship between oxidative stress and outcome/clinical severity of patients following aneurysmal subarachnoid hemorrhage (a common type of hemorrhagic stroke) and traumatic brain injury by detecting F2-isoprostanes, F4-neuroprostanes, multiple-lipid soluble antioxidants, and total nitrate/nitrite in plasma or cerebrospinal fluid from patients.
  3. The study of changes of primary antioxidant enzymes, tocopherols, and coenzyme Q10 in different grades of human astrocytoma tissues compared with non-tumor control tissues. 
  4. Detection of somatic mitochondrial DNA mutations by using direct sequencing, PCR-RFLP, denaturing HPLC, and pyrosequencing in different grades of human astrocytoma tissues and in recurrent tumor tissues.

 

 

近年來執行計畫 (Research Projects in Recent Years)
 
補助機構 (Funding sources)
CGMH Chang Gung Memorial Hospital; 長庚醫院
NSC/MOST :National Science Council/Ministry of Science and Technology; 國科會/科技部

 

補助機構
(Funding sorces)

     
(Name of Projects)

起迄年月
(Periods)

擔任
主持人(PI) /
共同主持人
(Co-PI)

CGMH

在以變性高效液相層析儀和Surveyor核酸酶偵測人類粒線體DNA時核內粒線體DNA假基因之干擾:證明與評估策略

2012.06~
2013.05

PI

Interference of mtDNA pseudogenes in the nucleus on detecting mutations of human mtDNA by denaturing HPLC and Surveyor nuclease: demonstration and evaluation strategy

NSC

粒線體氧化磷酸化抑制劑在人類細胞中造成輔酶Q10 缺乏之機制的探討

2012.08~
2013.07

PI

The study on the mechanisms of coenzyme Q10 deficiency induced by inhibitors of mitochondrial oxidative phosphorylation in human cells

NSC

人類COQ5蛋白在有內生性輔Q10含量變化之粒線體功能缺陷和氧化壓力狀況下所扮演之角色

2013.08~
2016.07

PI

The roles of human COQ5 protein in the conditions of mitochondrial dysfunction and oxidative stress with alterations in endogenous coenzyme Q10 levels

CGMH

含致病性粒線體DNA突變之cybrid中的輔酶Q10和不同COQ基因之狀態及COQ5基因改變之效應

2013.10~
2015.09

PI

Status of coenzyme Q10 and different COQ genes and the effect of modulation on the COQ5 gene in the cybrids with pathogenic mtDNA mutations

CGMH

人類細胞中各個PDSSCOQ蛋白在內生性輔酶Q10含量之維持及粒線體中COQ蛋白複合體之穩定性上所扮演的角色

2016.02~
2018.01

PI

The role of each PDSS and COQ protein in maintaining endogenous levels of coenzyme Q10 and stabilizing the COQ protein complex in the mitochondria in human cells

MOST

外加輔酶Q9或輔酶Q10對含有A8344G粒線體DNA突變之人類cybrid細胞中輔酶Q10含量、COQ蛋白及基因表現量、和COQ蛋白複合體之穩定性的影響

2016.08~
2017.07

PI

Effect of exogenous coenzyme Q9 or coenzyme Q10 on coenzyme Q10 levels, expression of COQ proteins and genes, and the stability of the COQ protein complex in human cybrids with A8344G mutation of mtDNA

MOST

外加輔酶QPDSSCOQ基因默化在人類細胞中所造成之內生性輔酶Q含量下降、COQ蛋白或COQ蛋白複合體不穩定、及粒線體功能異常的恢復作用

2017.08~
2020.07

PI

Restoration of endogenous coenzyme Q levels, stability of COQ proteins or COQ protein complex, and mitochondrial functions in human cells suppressed by the knockdown of PDSS or COQ genes after supplementation of exogenous coenzyme Q

CGMH

比較有無巴拉刈中毒小鼠之肺、心臟、及肝臟組織中脂質過氧化產物、脂溶性抗氧化物、及抗氧化酵素之含量

2018.02~

2019.07

PI

Comparison on the levels of lipid peroxidation products, lipid-soluble antioxidants, and antioxidant enzymes in lung, heart, and liver tissues from mice without versus with paraquat poisoning

CGMH

免疫抑制治療對巴拉刈中毒小鼠各組織中脂質過氧化程度及內生性coenzyme Q含量之影響

2019.08~

2022.01

PI

Effect of immunosuppressive therapy on the extent of lipid peroxidation and endogenous coenzyme Q levels in various tissues of paraquat-poisoned mice

MOST

人類細胞中各個PDSS和COQ蛋白之特性分析及其與粒線體功能失常或氧化壓力的關聯性

2020.08~

2023.07

PI
Characterization of various PDSS and COQ proteins in human cells and its relationship with mitochondrial dysfunction or oxidative stress

CGMH

巴拉刈中毒後特異性脂質過氧化指標F2-isoprostanes和脂溶性抗氧化物之偵測

 2017.07~

2019.06

Co-PI
(主持人為林口 長庚腎臟科顏宗海醫師)

Detection of specific lipid peroxidation marker F2-isoprostanes and lipid-soluble antioxidants in paraquat intoxication

CGMH

評估慢性鉛腎變之氧化壓力與發炎反應程度

2019.08~
2022.07

Co-PI
(主持人為林口 長庚腎臟科顏宗海醫師)
Level of oxidative stress and inflammation in chronic lead nephropathy
 

 

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