自由基暨粒線體醫學實驗室_研究方向 Research Direction
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Regulation of endogenous coenzyme Q10 (CoQ10) levels and human PDSS or COQ genes and proteins essential for biosynthesis of CoQ10 in response to oxidative stress or mitochondrial dysfunction in human cell lines or cybrids.
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Regulation of endogenous coenzyme Q10 (CoQ10) levels and human PDSS or COQ genes essential for the biosynthesis of coenzyme Q10 in response to oxidative stress or mitochondrial dysfunction in human cell lines or cybrids harboring pathogenic mutations of mitochondrial DNA (mtDNA).
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Characterization of human PDSS and COQ proteins and mouse Pdss and Coq proteins and identification of high-molecular-weight protein complexes in the mitochondria.
臨床及轉譯研究 (Clinical and Translational Research)
- Establishment of the only platform available in Taiwan to detect F2-isoprostanes, isofurans, and F4-neuroprostanes, which are the most specific markers of lipid peroxidation in vivo, in body fluids (CSF, plasma, urine), and tissues by the gas chromatography/negative-ion-chemical-ionization mass spectrometry (GC/NICI-MS) method for studying the relationship between various human conditions and oxidative damage. The research topics that have been applied this platform in collaboration with clinicians at the Linkou Chang Gung Memorial Hospital are aneurysmal subarachnoid hemorrhage (a common type of hemorrhagic stroke), traumatic brain injury, Alzheimer’s disease, paraquat (an herbicide) poisoning, and lead (Pb) poisoning.
- The investigation on the correlation between the changes related to primary antioxidant enzymes, CoQ10 , and somatic mtDNA mutations and grades or malignancy of human astrocytoma tissues.
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補助機構 |
計 畫 名 稱 |
起迄年月 |
擔任 |
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NSC |
人類COQ5蛋白在有內生性輔酶Q10含量變化之粒線體功能缺陷和氧化壓力狀況下所扮演之角色 |
2013.08~ |
PI |
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The roles of human COQ5 protein in the conditions of mitochondrial dysfunction and oxidative stress with alterations in endogenous coenzyme Q10 levels |
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CGMH |
含致病性粒線體DNA突變之cybrid中的輔酶Q10和不同COQ基因之狀態及COQ5基因改變之效應 |
2013.10~ |
PI |
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Status of coenzyme Q10 and different COQ genes and the effect of modulation on the COQ5 gene in the cybrids with pathogenic mtDNA mutations |
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| CGMH |
人類細胞中各個PDSS和COQ蛋白在內生性輔酶Q10含量之維持及粒線體中COQ蛋白複合體之穩定性上所扮演的角色 |
2016.02~ |
PI |
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The role of each PDSS and COQ protein in maintaining endogenous levels of coenzyme Q10 and stabilizing the COQ protein complex in the mitochondria in human cells |
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| MOST |
外加輔酶Q9或輔酶Q10對含有A8344G粒線體DNA突變之人類cybrid細胞中輔酶Q10含量、COQ蛋白及基因表現量、和COQ蛋白複合體之穩定性的影響 |
2016.08~ |
PI |
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Effect of exogenous coenzyme Q9 or coenzyme Q10 on coenzyme Q10 levels, expression of COQ proteins and genes, and the stability of the COQ protein complex in human cybrids with A8344G mutation of mtDNA |
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| MOST |
外加輔酶Q對PDSS或COQ基因默化在人類細胞中所造成之內生性輔酶Q含量下降、COQ蛋白或COQ蛋白複合體不穩定、及粒線體功能異常的恢復作用 |
2017.08~ |
PI |
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Restoration of endogenous coenzyme Q levels, stability of COQ proteins or COQ protein complex, and mitochondrial functions in human cells suppressed by the knockdown of PDSS or COQ genes after supplementation of exogenous coenzyme Q |
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| CGMH |
比較有無巴拉刈中毒小鼠之肺 |
2018.02~ 2019.07 |
PI |
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Comparison on the levels of lipid peroxidation products, lipid-soluble antioxidants, and antioxidant enzymes in lung, heart, and liver tissues from mice without versus with paraquat poisoning |
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| CGMH |
免疫抑制治療對巴拉刈中毒小鼠各組織中脂質過氧化程度及內生性coenzyme Q含量之影響 |
2019.08~ 2022.01 |
PI |
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Effect of immunosuppressive therapy on the extent of lipid peroxidation and endogenous coenzyme Q levels in various tissues of paraquat-poisoned mice |
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| MOST |
人類細胞中各個PDSS和COQ蛋白之特性分析及其與粒線體功能失常或氧化壓力的關聯性 |
2020.08~ 2023.07 |
PI |
| Characterization of various PDSS and COQ proteins in human cells and its relationship with mitochondrial dysfunction or oxidative stress | |||
| CGMH | 慢性鉛中毒後小鼠腎臟和血漿中脂質過氧化和 coenzyme Q狀態之變化及 Dimaval 螯合治療之可能保護作用 |
2022.10~ 2024.09 |
PI |
| Changes in the status of lipid peroxidation and coenzyme Q in mouse kidneys and plasma following chronic lead poisoning and the potential protective effect of the Dimaval chelation therapy | |||
| NSC | 小鼠細胞和組織之Pdss2和多個Coq蛋白的特性分析與含這些蛋白之蛋白複合體的鑑定 |
2023.08~ 2024.07 |
PI |
| Characterization of Pdss2 and multiple Coq proteins and identification of protein complexes containing these proteins in mouse cells and tissues | |||
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CGMH |
巴拉刈中毒後特異性脂質過氧化指標F2-isoprostanes和脂溶性抗氧化物之偵測 |
2017.07~ 2019.06 |
Co-PI
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Detection of specific lipid peroxidation marker F2-isoprostanes and lipid-soluble antioxidants in paraquat intoxication |
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| CGMH |
評估慢性鉛腎變之氧化壓力與發炎反應程度 |
2019.08~ |
Co-PI （主持人為林口 長庚腎臟科顏宗海醫師） |
| Level of oxidative stress and inflammation in chronic lead nephropathy |
