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張國友 教授

張 國 友 (Kowit Yu Chong)
張國友

學歷

美國南伊利諾大學藥理學博士

任課科目

高等生物技術學、生物技術實驗

研究專長

幹細胞治療、多重抗藥性癌細胞、再生醫學

辦公室分機

+886-3-2118800 X3332

+886-3-2118393

實驗室分機

+886-3-2118800 X3433

個人網頁

再生醫學研究室

E-mail

kchong@mail.cgu.edu.tw

研究方向及研究室特色(Lab & Research Interest)

1.  探討低氧預處理之幹細胞對Bleomycin誘導肺纖維化的小鼠之治療效果    

      特發性肺纖維化 (IPF) 目前被認為是一種原因不明之呼吸道彌漫性發炎性疾病，一連串發炎反應及間質細胞過度累積為造成肺部細胞功能異常之主要原因。研究發現當肺損傷所誘發之發炎反應，其炎性細胞所釋放之細胞激素會刺激大量產生，激活TGF-β1之訊號途徑及誘導膠原蛋白累積而導致肺纖維化。近來許多研究指出，利用肝細胞生長因子 (HGF) 之基因治療法有明顯改善經由Bleomycin所誘導之細胞凋亡、發炎反應及肺纖維化等病症。另外，目前利用間葉幹細胞 (MSC)治療肺損傷動物模式已有顯著成效，MSC除了降低傷害外亦針對損傷部位進行修復；但近來研究亦指出，移植之MSC容易受到損傷微環境之傷害而死亡，而造成應用MSC治療的障礙。再者，科學家利用預先給予低氧預處理(preconditioning) 之幹細胞治療心肌梗塞等疾病時，發現其成功改善移植後之幹細胞的存活率及增加治療疾病的效果。因此本研究擬探討低氧預處理之幹細胞在Bleomycin誘導肺纖維化的小鼠之療效。

2.  探討多重抗藥性癌細胞抗藥性的分子機制
      多重抗藥性癌細胞目前已成為臨床上以化療藥物治療惡性腫瘤的主要障礙。此種情形主要由於癌細胞細胞表面藥物通透性的改變而造成。儘管多重抗藥性抑制劑已被發現和研究多年，但始終無法有效應用於臨床上治療，尋找新穎的調控途徑來殺死多重抗藥性癌細胞成為當務之急。近年科學家發現一個以蛋白酶體抑制的新穎方法可以誘發癌細胞走向細胞凋亡。儘管許多研究已證實蛋白酶體抑制劑的療效。不過，仍有一部分的病人對於蛋白酶體抑制劑的治療失效。因此，我們假設這些病人體內的多重抗藥性癌細胞可能衍生出新穎機制來拮抗蛋白酶體抑制劑bortezomib的毒殺效果。

論文與著作(Publication)

Selected Publications (2018-2024)

  1. Tung YT, Chen HL, Wu HS, Ho MH, Chong KY, Chen CM. Kefir Peptides Prevent Hyperlipidemia and Obesity in High-Fat-Diet-Induced Obese Rats via Lipid Metabolism Modulation.  Mol Nutr Food Res. 62(3). doi: 10.1002/mnfr.201700505, 2018
  2. Chang YW, Tseng CP, Lee CH, Hwang TL, Chen YL, Su MT, Chong KY , Lan YW, Wu CC, Chen KJ, Lu FH, Liao HR, Hsueh C, Hsieh PW.  β-Nitrostyrene derivatives attenuate LPS-mediated acute lung injury via the inhibition of neutrophil-platelet interactions and NET release. Am J Physiol Lung Cell Mol Physiol. 314: L654-69, 2018.
  3. Huang TT, Lan YW, Ko YF, Chen CM, Lai HC, Ojcius DM, Martel J, Young JD, Chong KY*. Antrodia cinnamomea produces anti-angiogenic effects by inhibiting the VEGFR2 signaling pathway. J Ethnopharmacol. 220: 239-49, 2018.
  4. Hiew MSY, Cheng HP, Huang CJ, Chong KY, Cheong SK, Choo KB, Kamarul T. Incomplete cellular reprogramming of colorectal cancer cells elicits an epithelial/ mesenchymal hybrid phenotype. J Biomed Sci. 25: 57, 2018.
  5. Lee IT, Lin CF, Huang YL, Chong KY, Hsieh MF, Huang TH, Cheng CY. Protective mechanisms of resveratrol derivatives against TNF-α-induced inflammatory responses in rat mesangial cells. Cytokine. 113: 380-92, 2018.
  6. Huang TT, Lan YW, Chen CM, Ko YF, Ojcius DM, Martel J, Young JD, Chong KY*.  Antrodia cinnamomea induces anti-tumor activity by inhibiting the STAT3 signaling pathway in lung cancer cells.  Sci Rep. 9: 5145, 2019.
  7. Lan YW, Yang JC, Yen CC, Huang TT, Chen YC, Chen HL, Chong KY*, Chen CM*. Predifferentiated amniotic fluid mesenchymal stem cells enhance lung alveolar epithelium regeneration and reverse elastase-induced pulmonary emphysema. Stem Cell Res Ther. 10: 163, 2019.
  8. Chen HL, Hung KF, Yen CC, Laio CH, Wang JL, Lan YW, Chong KY, Fan HC, Chen, CM.  Kefir peptides alleviate particulate matter <4 μm (PM4.0)-induced pulmonary inflammation by inhibiting the NF-κB pathway using luciferase transgenic mice. Sci Rep 9: 11529, 2019.
  9. Yen CC, Chang WH, Tung MC, Chen HL, Liu HC, Liao CH, Lan YW, Chong KY, Yang SH, Chen CM. Lactoferrin Protects Hyperoxia-Induced Lung and Kidney Systemic Inflammation in an In Vivo Imaging Model of NF-κB/Luciferase Transgenic Mice. Mol Imaging Biol. 22: 526-38, 2020.
  10. Ou-Yang H, Wu SC, Sung LY, Yang SH, Yang SH, Chong KY, Chen CM. Article stat3 is an upstream regulator of granzyme g in the maternal-to-zygotic transition of mouse embryos. Int J Mol Sci. 22: 460, 2021
  11. Lin SS, Niu CC, Yuan LJ, Tsai TT, Lai PL, Chong KY, Wei KC, Huang CY, Lu ML, Yang CY, Ueng SWN. Mir-573 regulates cell proliferation and apoptosis by targeting Bax in human degenerative disc cells following hyperbaric oxygen treatment. J Orthop Surg Res. 16: 16, 2021.
  12. Tu MY, Han KY, Lan YW, Chang KY , Lai CW, Staniczek T, Lai CY, Chong KY*, Chen CM. Association of TGF-β1 and IL-10 Gene Polymorphisms with Osteoporosis in a Study of Taiwanese Osteoporotic Patients. Genes (Basel). 12(6): 930, 2021.
  13. Huang CH# , Chong KY# , Lei KF. Analysis of the Internal Hypoxic Environment in Solid Tumor Tissue Using a Folding Paper System. ACS Appl Mater Interfaces. 13(29):33885-33893, 2021
  14. Wang JL, Tsai YT, Lin CH, Cidem A, Staniczek T, Chang GRL, Yen CC, Chen W, Chong KY*, Chen CM.  Benefits of metformin combined with pemetrexed-based platinum doublets as a first-line therapy for advanced lung adenocarcinoma patients with diabetes. Biomolecules. 11(8):1252, 2021.
  15. Chen CF, Li HP, Chao YH, Tu MY, Yen CC, Lan YW, Yang SH, Chong KY*, Lin CC*, Chen CM*. Suppression of Dendritic Cell Maturation by Kefir Peptides Alleviates Collagen-Induced Arthritis in Mice. Front. Pharmacol. 12: 721594, 2021.
  16. Chen YC, Lan YW, Huang SM, Yen CC, Chen W, Wu WJ, Theresa Staniczek, Chong KY, Chen CM.  Human amniotic fluid mesenchymal stem cells attenuate pancreatic cancer cell proliferation and tumor growth in an orthotopic xenograft mouse model.  Stem Cell Res Ther. 13:235, 2022. 
  17. Liu YH, Chen YH, Ko CH, Kuo CW, Yen CC, Chen Wei, Chong KY*, and Chen CM*.  SOD3 and IL-18 Predict the First Kidney Disease-Related Hospitalization or Death during the One-Year Follow-Up Period in Patients with End-Stage Renal Disease. Antioxidants. 11, 1198. 2022.
  18. Wen YH, Lee CF, Chen YJ, Chang GJ* and Chong KY*.  Risks in Induction of Platelet Aggregation and Enhanced Blood Clot Formation in Platelet Lysate Therapy: A Pilot Study.  J Clin Med. 11: 3972, 2022.
  19. Huang TT, Chen CM, Chen LG, Lan YW, Huang TH, Choo KB and Chong KY*.  2,3,5,4'-tetrahydroxystilbene -2-O-β-D-glucoside ameliorates bleomycin-induced pulmonary fibrosis via regulating pro-fibrotic signaling pathways.  Front. Pharmacol. 13: 997100, 2022.
  20. Huang TT, Chen CM, Lan YW, Lin SS, Choo KB, Chong KY*. Blockade of c-Met-Mediated Signaling Pathways by E7050 Suppresses Growth and Promotes Apoptosis in Multidrug-Resistant Human Uterine Sarcoma Cells. Int J Mol Sci. 23: 14884, 2022.
  21. Huang TT, Chen CM, Lin SS, Lan YW, Cheng HC, Choo KB, Wang CC, Huang TH, Chong KY. E7050 Suppresses the Growth of Multidrug-Resistant Human Uterine Sarcoma by Inhibiting Angiogenesis via Targeting of VEGFR2-Mediated Signaling Pathways. Int J Mol Sci. 24(11): 9606, 2023.
  22. Lin SS, Ueng SWN, Chong KY, Chan YS, Tsai TT, Yuan LJ, Liu SJ, Yang CY, Hsiao HY, Hsueh YJ, Chen CA, Niu CC. Effects of Hyperbaric Oxygen Intervention on the Degenerated Intervertebral Disc: From Molecular Mechanisms to Animal Models. Cells. 12(16): 2111, 2023.
  23. Lan YW, Chen WR, Chang GR, Chen YC, Chong KY, Chuang KC, Kao YT, Chen MS, Chen CM. Aldo-keto reductase family 1 member A1 (AKR1A1) exerts a protective function in alcohol-associated liver disease by reducing 4-HNE accumulation and p53 activation. Cell Biosci. 14(1):18, 2024.
  24. Yen CC, Wu PY, Ou-Yang H, Chen HL, Chong KY, Chang RL, Chen CM. Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and AnticancerFunctionalities. Int J Mol Sci. 25(3):1818, 2024.
  25. Lan YW, Chen CE, Huang TT, Huang TH, Chen CM, Chong KY*. Antrodia cinnamomea extract alleviates bleomycin-induced pulmonary fibrosis in mice by inhibiting the mTOR pathway. Biomed J. 2024 , 100720.

Book Chapter

  1. Chan AWS, Chong KY and Schatten GP. Transgenic bovine embryo selection using green fluorescent protein. In: Hicks BW (ed) Green Fluorescent Protein: Method in Molecular biology 183. Humana Press, New Jersey, 2002, pp 201-214
  2. Chan AWS, Chong KY and Schatten GP. Production of Transgenic Nonhuman Primates. In Pinkert CA (ed) Transgenic Animal Technology, 2nd edn. Academic Press, San Diego, 2002. pp 359-394.4.   
  3. Lan YW, Chen CM, Chong KY. In Vitro Methods to Evaluate the Effects of Mesenchymal Stem Cells on TGF-β1-Induced Pulmonary Fibrosis.  In Vitro Models for Stem Cell Therapy. 2021, pp 83-92.

 

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