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Hsiu-Chuan Yen

Hsiu-Chuan Yen

Highest Degree

Ph.D. from Graduate Center for Toxicology, University of Kentucky, U.S.A.

Teaching Courses

(1) Undergraduate: clinical toxicology, clinical biochemistry, cell biology, clinical physiology; (2) Master: mitochondrial biology and medicine and its applications, advanced biotechnology; (3) Ph.D.: free Radical biology and medicine

Areas of Specialty

Free radical biology and medicine, mitochondrial biology and medicine, toxicology, clinical chemistry

Office Phone


Lab phone


Research website:




Lab & Research Interest

1. Establishment of the only platform available in Taiwan to detect F2-isoprostanes, isofurans, and F4-neuroprostanes, which are the most specific markers of lipid peroxidation in vivo, in biological fluids, tissues, or cells by gas chromatography/negative-ion-chemical-ionization mass spectrometry (GC/NICI-MS) reference method for studying the relationship between various human diseases and oxidative damage. The research topics that have been applied this platform in collaboration with clinicians at Linkou Chang Gung Memorial Hospitals are aneurysmal subarachnoid hemorrhage (a common type of hemorrhagic stroke), traumatic brain injury, Alzheimer’s disease, and paraquat (a herbicide) poisoning with immunosuppression therapy.

2. Regulation of endogenous coenzyme Q10 levels and human PDSS or COQ genes and proteins essential for biosynthesis of coenzyme Q10 in response to oxidative stress or mitochondrial dysfunction in human cell lines or cybrids.

3. Investigation on the stabilization of human PDSS or COQ protein-containing high-molecular-weight protein complex or the restoration of endogenous coenzyme Q10 levels by exogenous coenzyme Q under conditions of reduced expression of PDSS or COQ genes.

4. Investigation on the alterations of antioxidant enzymes, changes of coenzyme Q10 levels, and the presence of somatic mitochondrial DNA mutations in human astrocytoma tissues.


Journal articles in recent 5 years (Updated in 2018)

  1. S-W Chen, C-C Liu, H-C Yen*. 2013 March. Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5. Mitochondrion, 13: 143-152. [SCI]

  2. 顏秀娟*. 2013 June. 內生性輔酶Q之淺談. 生物醫學, 6:103-110.

  3. H-C Yen*, H-J Wei, and T-W Chen. 2013 July. Analytical variables affecting analysis of F2-isoprostanes and F4-neuroprostanes in human cerebrospinal fluids by gas chromatography/mass spectrometry. Biomedical Research International, 2013: Article 810915 (1-14). [SCI]

  4. H-C Yen*, S-L Li, W-C Hsu, P Tang. 2014 March. Interference of co-amplified nuclear mitochondrial DNA sequences on the determination of human mtDNA heteroplasmy by using the SURVEYOR Nuclease and the WAVE HS system. PLOS ONE, 9: e92817 (1-18). [SCI]

  5. H-C Yen*, C-C Liu, C-C, Kan, C-S Chen, H-R Wei. 2014 September. Suppression of coenzyme Q10 levels and the induction of multiple PDSS and COQ genes in human cells following oligomycin treatment. Free Radical Research, 48: 1125-1134. [SCI]

  6. H P Indo, H-C Yen, I Nakanishi, K-I Matsumoto, M Tamura, Y Nagano, H Matsui, O Gusev, R Cornette, T Okuda, Y Minamiyama, H Ichikawa, S Suenaga, M Oki, T Sato, T Ozawa, D K St.Clair, H J Majima*. 2015 January. A mitochondrial superoxide theory for oxidative stress diseases and aging. Journal of Clinical Biochemistry and Nutrition, 56: 1-7. [SCI]

  7. H-C Yen*, H-J Wei, C-L Lin. 2015 July. Unresolved issues in the analysis of F2-isoprostanes, F4-neuroprostanes, isofurans, neurofurans, and F2-dihomo-isoprostanes in body fluids and tissue using gas chromatography/negative-ion chemical-ionization mass spectrometry. Free Radical Research, 49: 861-880. [SCI]

  8. H-C Kuo*, H-C Yen, C-C Huang, W-C Hsu, H-J Wei, C-L Lin. 2015 October. Cerebrospinal fluid biomarkers for neuropsychological symptoms in early stage of late-onset Alzheimer's disease. International Journal of Neuroscience, 125: 747-754. [SCI]

  9. H-C Yen, T-W Chen, T-C Yang, H-J Wei, J-C Hsu*, C-L Lin*. 2015 December. Levels of F2-isoprostanes, F4-neuroprostanes, and total nitrate/nitrite in plasma and cerebrospinal fluid of patients with traumatic brain injury. Free Radical Research, 49: 1419-1430. [SCI]

  10.  HJ Majima*, HP Indo, I Nakanishi, S Suenaga, K Matsumoto, H Matsui, Y Minamiyama, H Ichikawa, H-C Yen, CL Hawkins, MJ Davies, T Ozawa, DK St Clair. 2016 March. Chasing great paths of Helmut Sies “Oxidative Stress”. Archives of Biochemistry and Biophysics, 595, 54-60. (SCI)

  11. H-C Yen*, Y-C Liu, C-C Kan, H-J Wei, S-H Lee, Y-H Wei, Y-H Feng, C-W Chen, C-C Huang. 2016 September. Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency. Biochimica et Biophysica Acta-General Subjects, 1860: 1864-1876. [SCI]

  12. H-C Yen*, C-L Lin, B-S Chen, C-W Chen, K-C Wei, M-L Yang, J-C Hsu, Y-H Hsu. 2018 August. Alterations of the levels of primary antioxidant enzymes in different grades of human astrocytoma tissues. Free Radical Research, 52: 856-871. [SCI]

(* corresponding author)