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Hsiu-Chuan Yen

Hsiu-Chuan Yen


Highest Degree

Ph.D. from Graduate Center for Toxicology, University of Kentucky, U.S.A.

Teaching Courses

(1) Undergraduate: clinical toxicology, clinical biochemistry, cell biology, clinical physiology; (2) Master: mitochondrial biology and medicine and its applications, advanced biotechnology; (3) Ph.D.: free Radical biology and medicine

Areas of Specialty

Free radical biology and medicine, mitochondrial biology and medicine, toxicology, clinical chemistry

Office Phone


Lab phone


Research website:

Free Radical and Mitochondrial Medicine Laboratory



Lab & Research Interest

1. Establishment of the only platform available in Taiwan to detect F2-isoprostanes, isofurans, and F4-neuroprostanes, which are the most specific markers of lipid peroxidation in vivo, in biological fluids, tissues, or cells by gas chromatography/negative-ion-chemical-ionization mass spectrometry (GC/NICI-MS) reference method for studying the relationship between various human diseases and oxidative damage. The research topics that have been applied this platform in collaboration with clinicians at Linkou Chang Gung Memorial Hospitals are aneurysmal subarachnoid hemorrhage (a common type of hemorrhagic stroke), traumatic brain injury, Alzheimer’s disease, and paraquat (a herbicide) poisoning with immunosuppression therapy.

2. Regulation of endogenous coenzyme Q10 levels and human PDSS or COQ genes and proteins essential for biosynthesis of coenzyme Q10 in response to oxidative stress or mitochondrial dysfunction in human cell lines or cybrids.

3. Investigation on the stabilization of human PDSS or COQ protein-containing high-molecular-weight protein complex or the restoration of endogenous coenzyme Q10 levels by exogenous coenzyme Q under conditions of reduced expression of PDSS or COQ genes.

4. Investigation on the alterations of antioxidant enzymes, changes of coenzyme Q10 levels, and the presence of somatic mitochondrial DNA mutations in human astrocytoma tissues.


Publications in recent years; updated on 2022/11

  1. HP Indo, HC Yen, I Nakanishi, KI Matsumoto, M Tamura, Y Nagano, H Matsui, O Gusev, R Cornette, T Okuda, Y Minamiyama, H Ichikawa, S Suenaga, M Oki, T Sato, T Ozawa, D K St.Clair, H J Majima*. A mitochondrial superoxide theory for oxidative stress diseases and aging. Journal of Clinical Biochemistry and Nutrition, 56: 1-7, 2015.

  2. HC Yen*, HJ Wei, CL Lin. Unresolved issues in the analysis of F2-isoprostanes, F4-neuroprostanes, isofurans, neurofurans, and F2-dihomo-isoprostanes in body fluids and tissue using gas chromatography/negative-ion chemical-ionization mass spectrometry. Free Radical Research, 49: 861-880, 2015.

  3. HC Kuo*, HC Yen, CC Huang, WC Hsu, HJ Wei, CL Lin. Cerebrospinal fluid biomarkers for neuropsychological symptoms in early stage of late-onset Alzheimer's disease. International Journal of Neuroscience, 125: 747-754, 2015.

  4. HC Yen, TW Chen, TC Yang, HJ Wei, JC Hsu*, CL Lin*. Levels of F2-isoprostanes, F4-neuroprostanes, and total nitrate/nitrite in plasma and cerebrospinal fluid of patients with traumatic brain injury. Free Radical Research, 49: 1419-1430, 2015.

  5.  HJ Majima*, HP Indo, I Nakanishi, S Suenaga, K Matsumoto, H Matsui, Y Minamiyama, H Ichikawa, HC Yen, CL Hawkins, MJ Davies, T Ozawa, DK St Clair. Chasing great paths of Helmut Sies “Oxidative Stress”. Archives of Biochemistry and Biophysics, 595, 54-60, 2016.

  6. HC Yen*, YC Liu, CC Kan, HJ Wei, SH Lee, YH Wei, YH Feng, CW Chen, CC Huang. Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency. Biochimica et Biophysica Acta-General Subjects, 1860: 1864-1876, 2016.

  7. HC Yen*, CL Lin, BS Chen, CW Chen, KC Wei, ML Yang, JC Hsu, YH Hsu. Alterations of the levels of primary antioxidant enzymes in different grades of human astrocytoma tissues. Free Radical Research, 52: 856-871, 2018.

  8. HC Yen*, WY Yeh, SH Lee, YH Feng, SL Yang. Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other’s stability. Biochim Biophys Acta-Bioenergetics, 2020:148192, 2020.

  9. HC Yen*, BS Chen, SL Yang, SY Wu, CW Chang, KC Wei, JC Hsu, YH Hsu, TH Yen, CL Lin*. 2022 February. Levels of coenzyme Q10 and several COQ proteins in human astrocytoma tissues are inversely correlated with malignancy. Biomolecules, 12: 336, 2022.

  10. TH Yen, CW Chang, HR Tsai, JF Fu, HC Yen*. Immunosuppressive therapies attenuate paraquat-induced renal dysfunction by suppressing inflammatory responses and lipid peroxidation. Free Radic Biol Med 191: 249-260, 2022.

(* corresponding author)

(Please find other previous publications on lab webpage.)