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Chiuon-Chian Chiou


Chiuan-Chian Chiou


Highest Degree

Ph. D., Life Science, National Tsing Hua University, Taiwan

Areas of Specialty

Reagent Development, Genotoxicity, Molecular Biology

Office Phone


Lab phone


Research website

Molecular Medicine and Reagent Develpment



Lab & Research Interest

Nucleic Acid Structure

We are interested in exploring the structure and behavior of nucleic acids and nucleic acid analogs, and apply these materials in the biomedical studies and clinical diagnostics. The main topics include: 1) peptide nucleic acid mediated PCR clamping; 2) detecting rare gene mutations using peptide nucleic acid probes; 3) development of triplex-formation oligonucleotide probes; 4) aptamer chemistry.

Nucleic Acid Detection in Liquid Biopsy

Application of circulating extracellular vesicles in the diagnosis of lung cancer

Secretory Pathway and Cancer Development

Nucleic Acid Biosensors


A.  Journal Articles

  1. Luo JD, Chan EC, Shih CL, Chen TL, Liang Y, Hwang TL, Chiou CC* (2006) Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe. Nucleic Acids Research,34(2):e12. (*Corresponding author) 
  2. Chiou CC*, Luo JD, Chen TL (2007) Single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe for the detection of rare mutations. Nature Protocols, 1(6):2604-12. (*Corresponding author) 
  3. Lo FS, Luo JD, Lee YJ, Shu SG, Kuo MT and Chiou CC* (2009). High resolution melting analysis for mutation detection for ptpn11 gene: Applications of this method for diagnosis of noonan syndrome. Clinica Chimica Acta, 409:75-77. (*Corresponding author) 
  4. Chiou CC, Chen TL, Tsao KC, Shih SR, Huang CG, Huang YL, and Chang CM (2010). Detection of pandemic (H1N1) 2009 influenza virus by allele discrimination. Clinica Chimica Acta, 411:1080-1083. 
  5. Chiou CC*, Chen SW, Luo JD, Chien YT (2011) Monitoring triplex DNA formation with fluorescence resonance energy transfer between a fluorophore-labeled probe and intercalating dyes. Analytical Biochemistry, 416:1-7. (*Corresponding author)
  6. Lin YH, Chiang CC, Wu MH, Pan TM, Luo JD, Chiou CC (2011) Solid-state sensor incorporated in microfluidic chip and magnetic-bead enzyme immobilization approach for creatinine and glucose detection in serum. Applied Physics Letters, 99:253704. 
  7.  Li YC, Chiou CC, Luo JD, Chen WJ, Su LC, Chang YF, Chang YS, Lai CS, Lee CC, Chou C (2012) Sensitive detection of unlabeled  oligonucleotides using a paired surface plasma waves biosensor. Biosensors and Bioelectronics, 35(1):342-8.
  8. Lin YT, Luo JD, Chiou CC, Yang CM, Wang CY, Chou C, Lai CS(2013) Detection of KRAS mutation by combination of polymerase chain reaction (PCR) and EIS sensor with new amino group functionalization. Sensors & Actuator B: Chemical., 186:374-379
  9. Lin YH, Wang SH, Wu MH, Pan TM, Lai CS, Luo JD, Chiou CC* (2013) Integrating solid-state sensor and microfluidic devices for glucose, Urea and creatinine detection based on enzyme-carrying alginate microbeads. Biosensors and Bioelectronics, 43(1): 328-335.
  10. Tsao KC, Chiou CC, Chen TL, Huang CG, Hsieh EF, Shih SR.(2014) Detection of low copies of drug-resistant influenza viral gene by a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe. J Microbiol Immunol Infect, 47:254-256.
  11. Shih CL, Luo JD, Chang JWC, Chen TL, Chien YT, Yu CJ, Chiou CC* (2015, Sep). Circulating messenger RNA profiling with microarray and next-generation sequencing: Cross-platform comparison. Cancer Genomics and Proteomics, 12(5), 223-230.  (*Corresponding author) 
  12. Shih CL, Chong KY, Hsu SC, Chien HJ, Ma CT, Chang JWC, Yu CJ, Chiou CC* (2016, Jan). Development of a magnetic bead-based method for the collection of circulating extracellular vesicles. New Biotechnology, 33(1), 116-122.  (*Corresponding author)
  13. Purwidyantri A, Chen CH, Hwang BJ, Luo JD, Chiou CC, Tian YC, Lin CY, Cheng CH, Lai CS (2016, Mar). Spin-coated Au-nanohole arrays engineered by nanosphere lithography for a Staphylococcus aureus 16S rRNA electrochemical sensor. Biosensors and Bioelectronics, 77, 1086-1094. 
  14. Lin, YT, Purwidyantri A, LuoJD, Chiou CC, Yang CM, Lo CH, Hwang TL, Yen TH, Lai CS (2016, May). Programming a nonvolatile memory-like sensor for KRAS gene sensing and signal enhancement. Biosensors and Bioelectronics, 7963-70. (SCI, 7/73). 
  15. Luo JD, Chang YJ, Chang CM, You JF, Wei PL, Chiou CC* (2016, Mar). GeneGazer: A toolkit integrating two pipelines for personalized profiling and biosignature identification. Cancer Genomics and Proteomics, 12(2),141-150. (*Corresponding author)
  16. Chen TL, Chang W-C, Hsieh JJ, Cheng HY, Chiou CC* (2016, Sep). A sensitive peptide nucleic acid probe assay for detection of BRAF V600 mutations in Melanoma .Cancer Genomics and Proteomics, 13(5), 381-386. (*Corresponding author)
  17. Chen TL, Chang, JW-C, Wang CL, Yang CT, Wang MC, Chiou CC* (2016, Nov). A primer and probe set for detecting multiple types of EGFR exon 19 deletions. Analytical Biochemistry, 513, 61-67. (*Corresponding author)
  18. Lo FS, Chen TL, Chiou CC*(2017 Nov).Detection of Rare Somatic GNAS mutation in mccune-albright syndrome using a novel peptide nucleic acid probe in a single tube. Molecules, 22(11), 1874. (*Corresponding author)
  19. Wang MC, Wang, CL, Chen TL, Chang JW-C, Lu JJ, Chang PY, Chiou CC (2017 Oct). Predicting outcomes of EGFR-targeted therapy in non-small cell lung cancer patients using pleural effusions samples and peptide nucleic acid probe assay. Clinical Chemistry and Laboratory Medicine ,55(12), 1979-1986. (*Corresponding author)
  20. Purwidyantri A, Chen CH, Chen LY, Chen CC, Luo JD, Chiou CC, Tian YC, Lin CY, Yang CM, Lai HC, Lai CS (2017 Apr).Speckled ZnO nanograss electrochemical sensor for staphylococcus epidermidis detection  Journal of The Electrochemical Society, 164 (6) B205-B211 

B.  Patents

  1. Chiou CC, Luo JD “Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor” US Patent granted, 2010 (US7803543)
  2. 邱全芊,陳泰龍,駱紀東:核酸分子檢測方法及檢測套組, 中華民國發明專利,I617667, 2018